Mouse Myosin Light Chain 2 Monoclonal Antibody | anti-MYL2 antibody

Myosin Light Chain 2, phosphorylated (Ser19) (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Cat. Num. AAA609646

• Gene Names: MYL2; MLC2; CMH10; DKFZp779C0562
• Reactivity: Bovine, Mouse, Porcine, Rat; Does not crossreact with the cardiac isoform of Myosin Light Chain 2.
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Ascites
• Synonyms: Myosin Light Chain 2; Monoclonal Antibody; phosphorylated (Ser19) (MLC2; Myosin Regulatory Light Chain; MRLC; RLC; LC20); Anti -Myosin Light Chain 2; anti-MYL2 antibody

• Host: Mouse
• Reactivity: Bovine, Mouse, Porcine, Rat; Does not crossreact with the cardiac isoform of Myosin Light Chain 2.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4i15
• Specificity: Recognizes endogenous levels of human Myosin Light Chain 2 (smooth muscle) at ~18kD only when phosphorylated at serine 19.
• Purity/Purification: Ascites
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MYL2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC); ; Other applications not tested.
• Application Notes: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. ; Immunofluorescence: 1:200; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser19 of human Myosine Light Chain 2 (smooth muscle).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from HEK293, C2C12, and C6 cells, untreated (-) or lamda phosphatase treated (+), using Myosin Light Chain 2 Antibody (upper) or MBS609646 (lower).)

Related Product Information for anti-MYL2 antibody

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulindependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

Product Categories/Family for anti-MYL2 antibody

Antibodies; Abs to Myosin

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI Accession #: NP_000423.2
• NCBI GenBank Nucleotide #: NM_000432.3
• UniProt Accession #: P10916; Q16123; Q14908; Q6IB42; Q7Z3Z1
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin, light chain 2, regulatory, cardiac, slow
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10; DKFZp779C0562
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin
• UniProt Gene Name: MYL2
• UniProt Entry Name: MLRV_HUMAN

NCBI Description

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq]

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; cardiac muscle contraction; post-embryonic development; muscle filament sliding

Disease: Cardiomyopathy, Familial Hypertrophic, 10