Rabbit anti-Human Estrogen Receptor, alpha Monoclonal Antibody | anti-ESR1 antibody

Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)

Cat. Num. AAA609358

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Estrogen Receptor; alpha; Monoclonal Antibody; phosphorylated (Ser118) (ER); Anti -Estrogen Receptor; anti-ESR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4H323
• Specificity: Recognizes phospho-estrogen receptor alpha (Ser118), Mr 66kD. Species Crossreactivity: Human. Others not tested. Predicted to crossreact with camel, mouse, porcine and Syrian hamster based on sequence homology.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100uM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-ESR1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:2000 detects phospho-estrogen receptor (Ser118) in RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant estrogen receptor phosphorylated in vitro with MAP Kinase 2/Erk2, active. Lysates from estradiol and EGF treated MCF-7 cells (lanes 1 and 2) or recombinant estrogen receptor protein phosphorylated with MAPK2/Erk2 (lanes 3 and 4) were resolved by electrophoresis and transferred to nitrocellulose. Following blocking of the membrane, the nitrocellulose was either treated with lambda phosphatase (lanes 1 and 3) or left untreated (lanes 2 and 4), then probed with anti-phospho- Estrogen Receptor (Ser118) (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated, synthetic peptide containing the sequence …QLpSPF… in which pS corresponds to phosphoserine at residue 118 of human estrogen receptor alpha. The immunizing sequence has 12/13 identical amino acids in camel, mouse, porcine, and Syrian hamster.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Lysates from estradiol and EGF treated MCF-7 cells (lanes 1 and 2) or recombinant estrogen receptor alpha protein phosphorylated with MAPK2/Erk2 (lanes 3 and 4) were either treated with lambda phosphatase (lanes 1 and 3) or left untreated (lanes 2 and 4), then probed with MBS609358 (1:2000 dilution).)

Product Categories/Family for anti-ESR1 antibody

Antibodies; Abs to Receptors; Estrogen

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 66,216 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha 3*,4,5,6,7*/822 isoform; estrogen receptor alpha delta 4*,5,6,7*/654 isoform; estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform; estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform; estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance