Mouse anti-Human SUMO-1 Activating Enzyme, Subunit 1 Monoclonal Antibody | anti-SAE1 antibody

SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, HSPC140, SUA1)

Cat. Num. AAA609222

• Gene Names: SAE1; AOS1; SUA1; UBLE1A; FLJ3091; HSPC140
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SUMO-1 Activating Enzyme; Subunit 1; Monoclonal Antibody; Subunit 1 (SAE1; AOS1; HSPC140; SUA1); Anti -SUMO-1 Activating Enzyme; anti-SAE1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8J145
• Specificity: Recognizes human SUMO-1 Activating Enzyme, Subunit 1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-SAE1 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human SUMO-1 Activating Enzyme, Subunit 1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-SAE1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 4885585
• NCBI GeneID: 10055
• NCBI Accession #: NP_005491.1
• NCBI GenBank Nucleotide #: NM_005500.2
• UniProt Accession #: Q9UBE0; O95717; Q9P020; B2RDP5
• Molecular Weight: 38,450 Da
• NCBI Official Full Name: SUMO-activating enzyme subunit 1 isoform a
• NCBI Official Synonym Full Names: SUMO1 activating enzyme subunit 1
• NCBI Official Symbol: SAE1
• NCBI Official Synonym Symbols: AOS1; SUA1; UBLE1A; FLJ3091; HSPC140
• NCBI Protein Information: SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme
• UniProt Protein Name: SUMO-activating enzyme subunit 1
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: SAE1
• UniProt Synonym Gene Names: AOS1; SUA1; UBLE1A
• UniProt Entry Name: SAE1_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM]

Uniprot Description

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus; cytosol

Molecular Function: protein C-terminus binding; SUMO activating enzyme activity; ATP-dependent protein binding; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification