PAPC, Oxidized Biochemical
PAPC, Oxidized (OxPAPC, Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine)
Synonyms
PAPC; Oxidized; Oxidized (OxPAPC; Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine); Oxidized biochemical
Form/Format
Dried OxPAPC prepared by air oxidation
Application Notes
Suitable for use in biological assays.
Dilution: Biological Assays: 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as negative unoxidized controls.
Dilution: Biological Assays: 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as negative unoxidized controls.
Biological Activity
Biological activities of OxPAPC are mediated by a variety of signal transduction mechanisms, including elevation of cAMP and Ca2+ levels, activation of MAP kinases, PI-3-kinase and small GTPases Rac-1 and Cdc42. OxPAPC-induced gene expression is mediated by transcription factors such as Egr-1, NFAT, CREB, NRF2, ATF4 but does not involve NFkB-dependent transcription.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for PAPC, Oxidized biochemical
Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (OxPAPC) is a prototypic biologically active oxidized phospholipid first isolated from LDL minimally modified by oxidation (MM-LDL). OxPAPC is an active principle of MM-LDL that mimics several pro- and anti-inflammatory effects induced by oxidized lipoproteins. Oxidation of PAPC generates a mixture of oxidized phospholipids containing either fragmented or full- length oxygenated sn-2 residues. The best-characterized oxidatively fragmented species contain a five- carbon sn-2 residue bearing omega-aldehyde or omega-carboxyl groups. Oxydation of arachidonic acid residue also produces phospholipids containing esterified isoprostanes. Both fragmented and full-length oxygenated species can regulate immune reactions. Pro-inflammatory effects of OxPAPC include stimulation of endothelial cells to bind monocytes and induction of tissue clotting factor, IL-8, MCP-1, G-CSF and other mediators of atherothrombosis. Anti- inflammatory effects of OxPAPC are mediated by induction of protective enzymes such as heme oxygenase-1 as well as suppression of innate immune responses to bacterial lipopolysaccharide (LPS) due to inhibition of LPS recognition by LPS-binding protein (LBP) and CD14. OxPAPC is active in vivo and was shown to protect mice in several models of acute inflammation induced by bacterial products. In addition, oxidized phospholipids present in OxPAPC are recognised by scavenger receptor CD36 and auto-antibodies present in patients with anti-phospholipid syndrome.
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Product Notes
The PAPC, Oxidized (Catalog #AAA6083020) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in biological assays. Dilution: Biological Assays: 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as negative unoxidized controls. Researchers should empirically determine the suitability of the PAPC, Oxidized for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAPC, Oxidized, Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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