Mouse Verotoxin I-B subunit Monoclonal Antibody
Verotoxin I-B subunit (Shiga-like, SLT-1b)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography
Synonyms
Verotoxin I-B subunit; Monoclonal Antibody; Verotoxin I-B subunit (Shiga-like; SLT-1b); Anti -Verotoxin I-B subunit (Shiga-like; anti-Verotoxin I-B subunit antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G300
Specificity
Recognizes the 1-B subunit of Verotoxin (Shiga-like toxin-1).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography
Purified by Protein G affinity chromatography
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-Verotoxin I-B subunit antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA and Inhibition.
Dilution: Western Blot: 1:10
Inhibition: in vitro dilutions should be made according to the amount of toxin to be inactiviated.
Dilution: Western Blot: 1:10
Inhibition: in vitro dilutions should be made according to the amount of toxin to be inactiviated.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Verotoxin I-B subunit antibody
Verotoxins are also called Shiga-like toxins (SLTs). Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share > 99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
Product Categories/Family for anti-Verotoxin I-B subunit antibody
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Product Notes
The Verotoxin I-B subunit (Catalog #AAA608228) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Verotoxin I-B subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA and Inhibition. Dilution: Western Blot: 1:10 Inhibition: in vitro dilutions should be made according to the amount of toxin to be inactiviated. Researchers should empirically determine the suitability of the Verotoxin I-B subunit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Verotoxin I-B subunit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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