Western Blot (WB)
(Western Blot using MBS608109.)
Mouse Transglutaminase II Monoclonal Antibody
Transglutaminase II (BSA & Azide Free)
Reactivity
Canine, Guinea Pig, Human, Rabbit
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Transglutaminase II; Monoclonal Antibody; Transglutaminase II (BSA & Azide Free); Anti -Transglutaminase II (BSA & Azide Free); anti-Transglutaminase II antibody
Host
Mouse
Reactivity
Canine, Guinea Pig, Human, Rabbit
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F382
Specificity
Recognizes guinea pig Transglutaminase II. Species Crossreactivity: human, canine and rabbit Does not react with mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T8260-04
Applicable Applications for anti-Transglutaminase II antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Affinity Purification, Biological Blockade.
Dilution: Western Blot: 1-2ug/ml for 2 hours ar RT
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: 1-2ug/ml for 2 hours ar RT
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by researcher.
Immunogen
Puri?ed guinea pig liver TGase II. MW of Antigen: 77-85kD. Epitope: aa447-478. Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix
Positive Control
HUVEC cells. Placenta
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot using MBS608109.)
Western Blot (WB)
(Western Blot using MBS608109.)
Western Blot (WB)
(IP/Western Blot using MBS608109.)
Western Blot (WB)
(IP/Western Blot using MBS608109.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with MBS608109 using peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining of tumor and endothelial cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with MBS608109 using peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining of tumor and endothelial cells.)
Related Product Information for anti-Transglutaminase II antibody
Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-, cellular- transglutaminase. Different tissues and cell types express varying amounts of tissue transglutaminase with a markedly high expression in rheumatoid lesions. It is implicated in programmed cell death, signal transduction, drug resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
Product Categories/Family for anti-Transglutaminase II antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Transglutaminase II (Catalog #AAA608109) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Transglutaminase II (BSA & Azide Free) reacts with Canine, Guinea Pig, Human, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Transglutaminase II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Affinity Purification, Biological Blockade. Dilution: Western Blot: 1-2ug/ml for 2 hours ar RT Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Transglutaminase II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transglutaminase II, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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