Mouse anti-Human Ubiquitin Conjugating Enzyme E2L6 Monoclonal Antibody | anti-UBE2I antibody

Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)

Cat. Num. AAA607995

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ubiquitin Conjugating Enzyme E2L6; Monoclonal Antibody; Ubiquitin Conjugating Enzyme E2L6 (UBE2L6; UbcH8); Anti -Ubiquitin Conjugating Enzyme E2L6 (UBE2L6; anti-UBE2I antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 10C318
• Specificity: Recognizes human UBE2L6 (1-152aa)
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-UBE2I antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000-1:3000; immunohistochemistry: 1:50-1:100
• Immunogen: Recombinant human UBE2L6 (1-152aa) purified from E. coli
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-UBE2I antibody

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Product Categories/Family for anti-UBE2I antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 973255
• NCBI GeneID: 7329
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69; Q7KZS0
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: ubiquitin conjugating enzyme
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9; OTTHUMP00000045512; OTTHUMP00000158627; OTTHUMP00000158628; OTTHUMP00000158629; OTTHUMP00000198126; OTTHUMP00000198127; OTTHUMP00000198131; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

UBC9: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation (Probable). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML- RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: SUMO conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase; Nuclear receptor co-regulator; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; fibrillar center; synapse; synaptonemal complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; ubiquitin protein ligase binding; bHLH transcription factor binding; transcription factor binding; HLH domain binding; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; mitosis; viral reproduction; positive regulation of steroid hormone receptor signaling pathway; protein ubiquitination; protein modification process; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; chromosome segregation; cellular protein metabolic process; protein sumoylation; cell division; positive regulation of transcription factor activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent