Mouse anti-Human Suppressor of RNA Polymerase B Monoclonal Antibody | anti-MED21 antibody

Suppressor of RNA Polymerase B (SRB7)

Cat. Num. AAA607871

• Gene Names: MED21; SRB7; SURB7
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Suppressor of RNA Polymerase B; Monoclonal Antibody; Suppressor of RNA Polymerase B (SRB7); Anti -Suppressor of RNA Polymerase B (SRB7); anti-MED21 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9F642
• Specificity: Recognizes human SRB7
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-MED21 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:8000; Western Blot: 1:8000
• Immunogen: Recombinant human SRB7 (15kD)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-MED21 antibody

This complex associates with the RNA polymerase II holoenzyme through SRB7 to enhance the gene-specific activation or repression induced by DNA-binding transcription factors.

Product Categories/Family for anti-MED21 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 119616953
• NCBI GeneID: 9412
• UniProt Accession #: Q13503; Q6IB05; Q92811; B2R4I3
• Molecular Weight: 15,564 Da
• NCBI Official Full Name: SRB7 suppressor of RNA polymerase B homolog (yeast), isoform CRA_b
• NCBI Official Synonym Full Names: mediator complex subunit 21
• NCBI Official Symbol: MED21
• NCBI Official Synonym Symbols: SRB7; SURB7
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 21; hSrb7; OTTHUMP00000240653; OTTHUMP00000240654; RNAPII complex component SRB7; RNA polymerase II complex component SRB7; RNA polymerase II holoenzyme component SRB7; SRB7 suppressor of RNA polymerase B homolog
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 21
• Protein Family: Putative mediator of RNA polymerase II transcription
• UniProt Gene Name: MED21
• UniProt Synonym Gene Names: SRB7; SURB7
• UniProt Entry Name: MED21_HUMAN

NCBI Description

The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for transcriptional activation of polymerase II (see MIM 180660)-transcribed genes. MED21 is a Mediator subunit predicted to reside in the middle module and appears to be a core subunit found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM]

Uniprot Description

MED21: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 21 family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12p11.23

Cellular Component: Srb-mediator complex

Molecular Function: protein binding; DNA-directed RNA polymerase activity; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; blastocyst development; transcription, DNA-dependent; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter