Chemiluminescence
((0.03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Glucose Regulated Protein 94 Polyclonal Antibody | anti-HSP90B1 antibody
Glucose Regulated Protein 94 (Grp94, gp96, ECGP, Endoplasmin, Heat Shock Protein 90kD beta Member 1, HSP90b1, Stress Inducible Tumor Rejection Antigen gp96, Tumor Rejection Antigen 1, TRA1)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:32,000
Western Blot: 0.01-0.03ug/ml, observed in lysates of mouse and rat liver and of cell lines NIH3T3 and NSO on ~100kD bands
Immunohistochemistry (Paraffin): 3-5ug/ml
Chemiluminescence
((0.03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]
Uniprot Description
GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.
Protein type: Secreted; Heat shock protein; Apoptosis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q24.2-q24.3
Cellular Component: endoplasmic reticulum membrane; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; cytosol; extracellular matrix; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; midbody; nucleus
Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding
Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; unfolded protein response; regulation of phosphoprotein phosphatase activity; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sequestering of calcium ion; response to hypoxia; toll-like receptor signaling pathway; innate immune response; negative regulation of apoptosis
Research Articles on HSP90B1
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA607676) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Glucose Regulated Protein 94 (Grp94, gp96, ECGP, Endoplasmin, Heat Shock Protein 90kD beta Member 1, HSP90b1, Stress Inducible Tumor Rejection Antigen gp96, Tumor Rejection Antigen 1, TRA1) reacts with Bovine, Canine, Human, Mouse, Porcine, Rat0 and may cross-react with other species as described in the data sheet. AAA Biotech's Glucose Regulated Protein 94 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:32,000 Western Blot: 0.01-0.03ug/ml, observed in lysates of mouse and rat liver and of cell lines NIH3T3 and NSO on ~100kD bands Immunohistochemistry (Paraffin): 3-5ug/ml. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose Regulated Protein 94, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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