Mouse Neurofilament, 160kD Monoclonal Antibody | anti-NEFL antibody

Neurofilament, 160kD

Cat. Num. AAA607545

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E
• Reactivity: Bovine, Guinea Pig, Hamster, Human, Monkey, Rabbit, Xenopus
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: Neurofilament; 160kD; Monoclonal Antibody; Anti -Neurofilament; anti-NEFL antibody

• Host: Mouse
• Reactivity: Bovine, Guinea Pig, Hamster, Human, Monkey, Rabbit, Xenopus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6G5
• Specificity: Reacts exclusively with the phosphorylated isoform of the 160kD neurofilament protein.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-NEFL antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry.; Dilution: Flow Cytometry: 1:50-1:100; Immunohistochemistry: 1:50-1:100; Western Blot: 1:100-1:500
• Immunogen: Neurofilament preparation of calf brain tissue.
• Hybridoma: Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NEFL antibody

Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in

Product Categories/Family for anti-NEFL antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 105990539
• NCBI GeneID: 4747
• NCBI Accession #: NP_006149.2
• NCBI GenBank Nucleotide #: NM_006158.4
• UniProt Accession #: P07196; Q16154; Q8IU72; B9ZVN2
• Molecular Weight: 61,517 Da
• NCBI Official Full Name: neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E
• NCBI Protein Information: neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein
• UniProt Protein Name: Neurofilament light polypeptide
• Protein Family: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Uniprot Description

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein C-terminus binding; protein binding, bridging; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f