Mouse DYRK1A Monoclonal Antibody | anti-DYRK1A antibody

DYRK1A (Protein kinase minibrain homolog, MNBH, HP86, Dual specificity YAK1-related kinase, hMNB)

Cat. Num. AAA607359

• Gene Names: DYRK1A; MNB; DYRK; HP86; MNBH; DYRK1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: DYRK1A; Monoclonal Antibody; DYRK1A (Protein kinase minibrain homolog; MNBH; HP86; Dual specificity YAK1-related kinase; hMNB); Anti -DYRK1A (Protein kinase minibrain homolog; anti-DYRK1A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G351
• Specificity: Recognizes human protein kinase minibrain homolog. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-DYRK1A antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and direct ELISA.
• Immunogen: Partial recombinant protein of human protein kinase minibrain homolog (aa 674 to 763) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis on HeLa nuclear extract lysate using MBS607359.)

Related Product Information for anti-DYRK1A antibody

Protein kinase minibrain homolog phosphorylates serine, threonine and tyrosine residues in its sequence as well as in exogenous substrates. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. There are 5 named isoforms produced by alternative splicing. It is expressed ubiquitously with highest levels in skeletal muscle, testis, fetal lung and kidney, and in the developing central nervous system. It localises to the nucleus speckle. This protein is overexpressed 1.5-fold in fetal Down syndrome brain and considered a strong candidate for learning defects associated with Down syndrome. It belongs to the protein kinase superfamily, CMGC ser/Thr protein kinase family and MNB/DYRK subfamilly.

Product Categories/Family for anti-DYRK1A antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18765750
• NCBI GeneID: 1859
• NCBI Accession #: NP_567824.1
• NCBI GenBank Nucleotide #: NM_101395.2
• UniProt Accession #: O60770; O94789; Q13627; Q15446; Q76N25
• Molecular Weight: 6,810 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 3
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• NCBI Official Symbol: DYRK1A
• NCBI Official Synonym Symbols: MNB; DYRK; HP86; MNBH; DYRK1
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A; hMNB; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; MNB/DYRK protein kinase; serine/threonine kinase MNB; mnb protein kinase homolog hp86; protein kinase minibrain homolog; dual specificity YAK1-related kinase; serine/threonine-specific protein kinase
• UniProt Protein Name: MNB protein kinase
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Entry Name: O60770_HUMAN

NCBI Description

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq]

Uniprot Description

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); CMGC group; DYRK family; Dyrk1 subfamily

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm; nuclear speck; ribonucleoprotein complex; nucleus

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein self-association; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; tau protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; negative regulation of DNA damage response, signal transduction by p53 class mediator; nervous system development; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitotic cell cycle; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome

Disease: Mental Retardation, Autosomal Dominant 7