Rat anti-Mouse Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 Monoclonal Antibody

Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1, CD209 antigen-like protein B)

Cat. Num. AAA606657

• Reactivity: Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1; Monoclonal Antibody; Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1; CD209 antigen-like protein B); Anti -Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1; anti-Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 9G266
• Specificity: Recognizes murine SIGN-related 1 (SIGN-R1). Does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. MZM of the spleen are involved in the clearance of polysaccharides.
• Purity/Purification: Purified; Purified
• Form/Format: Sterile culture medium with a low endotoxin level.

• Applicable Applications for anti-Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Functional Blocking Studies, and Immunohistochemistry.; Dilution: Flow Cytometry: 1:50; Immunohistochesmitry (frozen and paraffin): 1:50; For blocking studies in vitro, dilutions have to be made according to the amounts of SIGN-R1 to; be inactivated. Optimal dilutions to be determined by the researcher.
• Immunogen: Mouse SIGN-R1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 antibody

SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. Mouse SIGN-R1 is a C-type lectin, like DC-SIGN which is expressed on human dendritic cells (DCs). However, mouse SIGN-R1 itself is not expressed on DCs. SIGN-R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN-R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody. Therefore, the monoclonal antibody ER-TR9 can be used to study the uptake of polysaccharides by macrophages.

Product Categories/Family for anti-Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 antibody

Antibodies; Abs to Lectins