Mouse anti-Human LOX-1, Soluble form Monoclonal Antibody | anti-Olr1 antibody

LOX-1, Soluble form (CLEC8A, hLOX-1, Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX1, LOX-1, oxidised low density lipoprotein (lectin-like) receptor 1, Oxidized low-density lipoprotein receptor

Cat. Num. AAA606030

• Gene Names: Olr1; LOX-1; Oldr1; Oldlr1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: LOX-1; Soluble form; Monoclonal Antibody; Soluble form (CLEC8A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; oxidised low density lipoprotein (lectin-like) receptor 1; Oxidized low-density lipoprotein receptor; Anti -LOX-1; anti-Olr1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9L453
• Specificity: Recognizes human recombinant LOX-158-273.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Olr1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Recombinant LOX-1 expressed in E. coli.
• Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Olr1 antibody

LOX-1 belongs to the C-type lectin-like protein superfamily. This unique scavenger receptor plays important roles in atherogenesis. Like other scavenger receptors, LOX-1 has a wide spectrum of physiological ligands, including OxLDL, anionic phospholipids, aged/apoptotic cells, activated platelets, and bacteria. The LOX-1 gene is a so-called immediate early gene that is dynamically modulated by several factors in vitro and in vivo. LOX-1 expression is induced by stimuli such as inflammatory cytokines, OxLDL, TNF-alpha, TGF-beta, and ANG II in vitro, and several proatherogenic factors in vivo. Monoclonal antibody neutralizes LOX-1 and inhibits Hsp70 binding to dendritic cells and Hsp70-induced antigen cross-presentation. In vivo, targeting LOX-1 with a tumor antigen using anti-LOX-1 antibody induces antitumor immunity.

Product Categories/Family for anti-Olr1 antibody

Antibodies; Abs to Leukotrienes

NCBI and Uniprot Product Information

• NCBI GI #: 4008187
• NCBI GeneID: 140914
• UniProt Accession #: O70156
• Molecular Weight: 41,890 Da
• NCBI Official Full Name: lectin-like oxidized low-density lipoprotein receptor
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; Oldr1; Oldlr1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density lipoprotein receptor-1; oxidised low density lipoprotein (lectin-like) receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• Protein Family: Oxidized low-density lipoprotein receptor
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1
• UniProt Entry Name: OLR1_RAT

NCBI Description

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Uniprot Description

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.2 Ref.4

Subunit structure: Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70

By similarity.

Subcellular location: Cell membrane; Single-pass type II membrane protein

By similarity. Secreted

By similarity. Note: A secreted form also exists

By similarity.

Tissue specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells. Ref.1

Induction: By hypertension. Up-regulated by shear stress, lipopolysaccharide and TNF-alpha in cultured vascular endothelial cells. Ref.1 Ref.2

Domain: The cytoplasmic region is required for subcellular sorting on the cell surface

By similarity.The C-type lectin domain mediates the recognition and binding of oxLDL

By similarity.The Neck region contains 3 internal repeats that are only found in rodents.

Post-translational modification: N-glycosylated

By similarity.

Sequence similarities: Contains 1 C-type lectin domain.