Mouse anti-Human Endostatin Monoclonal Antibody | anti-COL18A1 antibody

Endostatin

Cat. Num. AAA605990

• Gene Names: COL18A1; KS; KNO; KNO1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Endostatin; Monoclonal Antibody; Anti -Endostatin; anti-COL18A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9G184
• Specificity: Recognizes human endostatin, a 20kD C-terminal fragment of collagen XVIII.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-COL18A1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Dilution: Flow Cytometry: 1:10; Western Blot: 1:10; Immunohistochemistry (Frozen/paraffin): 1:10. Antigen retrieval using microwave irradiation (2x7 minutes) in citrate buffer is recommended for staining of paraffin sections.
• Immunogen: Recombinant human Endostatin
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-COL18A1 antibody

Endostatin is able to evoke non-uniform response for proliferation, cell mount and migration of entothelial cells, with different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce acquired drug resistance and has therefore become a potent new therapy strategy in solid neoplasias. This therapy appear to have high potential not only for the treatment of gliomas, the most common brain tumors, but also of other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn2+ binding and elastase processing. Widespread endostatin expression was found in elastic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.

Product Categories/Family for anti-COL18A1 antibody

Antibodies; Abs to Angiogenesis

NCBI and Uniprot Product Information

• NCBI GI #: 13957730
• NCBI GeneID: 80781
• UniProt Accession #: P39060; Q58EX6; Q6RZ39; Q6RZ40; Q6RZ41; Q8N4S4; Q8WXI5; Q96T70; Q9UK38; Q9Y6Q7; Q9Y6Q8; A8MVI4
• Molecular Weight: 178,188 Da
• NCBI Official Full Name: endostatin variant
• NCBI Official Synonym Full Names: collagen, type XVIII, alpha 1
• NCBI Official Symbol: COL18A1
• NCBI Official Synonym Symbols: KS; KNO; KNO1
• NCBI Protein Information: collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; antiangiogenic agent; multi-functional protein MFP
• UniProt Protein Name: Collagen alpha-1(XVIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL18A1
• UniProt Entry Name: COIA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity

Biological Process: response to drug; collagen catabolic process; negative regulation of cell proliferation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; organ morphogenesis; visual perception; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration

Disease: Knobloch Syndrome 1