Mouse anti-E Coli Cytotoxic Necrotizing Factor 1 Monoclonal Antibody
Cytotoxic Necrotizing Factor 1 (CNF1)
Reactivity
E Coli
Applications
Immunohistochemistry, Western Blot
Purity
Protein G
Synonyms
Cytotoxic Necrotizing Factor 1; Monoclonal Antibody; Cytotoxic Necrotizing Factor 1 (CNF1); Anti -Cytotoxic Necrotizing Factor 1 (CNF1); anti-Cytotoxic Necrotizing Factor 1 antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9G169
Specificity
Specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. Recognizes an epitope between aa704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
Purity/Purification
Protein G
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-Cytotoxic Necrotizing Factor 1 antibody
Immunohistochemistry - Frozen (IHC-F); Immuno Assays (IA); Western Blot (WB).
Application Notes
Dilutions to be usded depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working diultion is 1:10.
FS: For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.
FS: For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.
Immunogen
Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic E. coli.
Endotoxin
15.5 EU/mg
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cytotoxic Necrotizing Factor 1 antibody
CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis
Product Categories/Family for anti-Cytotoxic Necrotizing Factor 1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
cytotoxic necrotizing factor 1
Similar Products
Product Notes
The Cytotoxic Necrotizing Factor 1 (Catalog #AAA605262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytotoxic Necrotizing Factor 1 (CNF1) reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's Cytotoxic Necrotizing Factor 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry - Frozen (IHC-F); Immuno Assays (IA); Western Blot (WB). Dilutions to be usded depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working diultion is 1:10. FS: For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated. Researchers should empirically determine the suitability of the Cytotoxic Necrotizing Factor 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytotoxic Necrotizing Factor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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