anti-Human Hypoxia Inducible Factor 1a Monoclonal Antibody | anti-HIF1A antibody
Anti - Hypoxia Inducible Factor 1a (HIF-1a)
Gene Names
HIF1A; HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Hypoxia Inducible Factor 1a; Monoclonal Antibody; Anti - Hypoxia Inducible Factor 1a (HIF-1a); Hypoxia Inducible Factor 1a (HIF-1a); anti-HIF1A antibody
Host
Host: Mouse
Source: Human
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7C37
Specificity
Recognizes human Hypoxia Inducible Factor 1a.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Concentration
~1mg/ml (varies by lot)
Sequence Length
826
Applicable Applications for anti-HIF1A antibody
ELISA, Western Blot
Application Notes
Suitable for use in Western Blot and ELISA. Other applications not tested.
Dilution: Western Blot: 1:200-1:2000 using Chemiluminescence technique. Detected ~120kD bands representing post translationally modified forms of HIF-1a. Mammalian cells cultured under reduced (hypoxic) conditions.
ELISA: 1:10,000-1:30,000
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:200-1:2000 using Chemiluminescence technique. Detected ~120kD bands representing post translationally modified forms of HIF-1a. Mammalian cells cultured under reduced (hypoxic) conditions.
ELISA: 1:10,000-1:30,000
Optimal dilutions to be determined by the researcher.
Immunogen
Recombinant human purified HIF-1 alpha protein (aa530-826, GST fusion protein)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-HIF1A antibody
HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis, the posttranslational modification by prolyl hydroxylation as a key regulatory event that targets HIF-alpha subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The transcriptional complex is composed of an alpha-beta heterodimer; HIF-beta being a constitutive nuclear protein that dimerises with oxygen regulated HIF-alpha subunits. In normoxia, 4-hydroxylation of human HIF-alpha at Pro402 or Pro564 by a set of HIF prolyl hydroxylase isoenzymes (PHD 1-3) mediates HIF1-alpha recognition by von Hippel-Lindau ubiquitin ligase complex leading to its proteasomal destruction. In hypoxia (deprivation of oxygen), lack of hydroxylase activity enables HIF-alpha subunits to escape destruction and become transcriptionally active. Thus HIF hydroxylases provide a focus for understanding cellular responses to hypoxia and target for therapeutic manipulation. There are several HIF factors, which include HIF 1-alpha, HIF 1-beta, HIF 2-alpha
Product Categories/Family for anti-HIF1A antibody
References
• Talks et al. Am J Pathol 157: 411-421; Kaelin, W. G et al (1999) Nature 399, 203; Iyer, N. V et al (1998) Genes and Dev. 12, 149; Semenza, G. L et al (1998) J Lab Clin. Med. 131, 207.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
92,670 Da
NCBI Official Full Name
Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA
NCBI Protein Information
hypoxia-inducible factor 1-alpha; HIF-1-alpha; OTTHUMP00000179061; OTTHUMP00000179062; OTTHUMP00000179063; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
Protein Family
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF1A hif1a (Catalog #AAA604029) is an Antibody produced from Host: Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Hypoxia Inducible Factor 1a (HIF-1a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hypoxia Inducible Factor 1a can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in Western Blot and ELISA. Other applications not tested. Dilution: Western Blot: 1:200-1:2000 using Chemiluminescence technique. Detected ~120kD bands representing post translationally modified forms of HIF-1a. Mammalian cells cultured under reduced (hypoxic) conditions. ELISA: 1:10,000-1:30,000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypoxia Inducible Factor 1a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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