Flow Cytometry (FC/FACS)
(Flow Cytometry detection of SSEA‑4 in NTera‑2 human cell line using MBS604020.)
Mouse anti-Human, Mouse Stage Specific Embryonic Antigen 4 Monoclonal Antibody
Stage Specific Embryonic Antigen 4 (SSEA4)
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Stage Specific Embryonic Antigen 4; Monoclonal Antibody; Stage Specific Embryonic Antigen 4 (SSEA4); Anti -Stage Specific Embryonic Antigen 4 (SSEA4); anti-Stage Specific Embryonic Antigen 4 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
8H218
Specificity
Recognizes a carbohydrate epitope of human SSEA-4. Species Crossreactivity: Mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a lyophilized powder in TBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Applicable Applications for anti-Stage Specific Embryonic Antigen 4 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry, Immunohistochemistry
Dilution: Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10ug/mL.
Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Dilution: Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10ug/mL.
Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Immunogen
Human embryonal carcinoma cell line 2102 Ep
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the human embryonal carcinoma cell line 2102 Ep.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Flow Cytometry (FC/FACS)
(Flow Cytometry detection of SSEA‑4 in NTera‑2 human cell line using MBS604020.)
Flow Cytometry (FC/FACS)
(Flow Cytometry detection of SSEA‑4 in NTera‑2 human cell line using MBS604020.)
Immunocytochemistry (ICC)
(Immunocytochemistry of SSEA‑4 in BG01V human stem cells using MBS604020.)
Immunocytochemistry (ICC)
(Immunocytochemistry of SSEA‑4 in BG01V human stem cells using MBS604020.)
Related Product Information for anti-Stage Specific Embryonic Antigen 4 antibody
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.1-4
Product Categories/Family for anti-Stage Specific Embryonic Antigen 4 antibody
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Product Notes
The Stage Specific Embryonic Antigen 4 (Catalog #AAA604020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Stage Specific Embryonic Antigen 4 (SSEA4) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Stage Specific Embryonic Antigen 4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Flow Cytometry, Immunohistochemistry Dilution: Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10ug/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Researchers should empirically determine the suitability of the Stage Specific Embryonic Antigen 4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stage Specific Embryonic Antigen 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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