Mouse anti-Human NFkB Repressing Factor Monoclonal Antibody | anti-NKRF antibody

NFkB Repressing Factor (NKRF, ITBA4, NRF)

Cat. Num. AAA603882

• Gene Names: NKRF; NRF; ITBA4
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NFkB Repressing Factor; Monoclonal Antibody; NFkB Repressing Factor (NKRF; ITBA4; NRF); Anti -NFkB Repressing Factor (NKRF; anti-NKRF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8J130
• Specificity: Recognizes human NFkB Repressing Factor.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-NKRF antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human NFkB Repressing Factor
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-NKRF antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 63003897
• NCBI GeneID: 55922
• NCBI Accession #: NP_060014.2
• NCBI GenBank Nucleotide #: NM_017544.3
• UniProt Accession #: O15226; Q4VC41; Q9UJ91; Q6NUN2
• Molecular Weight: 77,673 Da
• NCBI Official Full Name: NF-kappa-B-repressing factor isoform 2
• NCBI Official Synonym Full Names: NFKB repressing factor
• NCBI Official Symbol: NKRF
• NCBI Official Synonym Symbols: NRF; ITBA4
• NCBI Protein Information: NF-kappa-B-repressing factor; OTTHUMP00000023906; OTTHUMP00000023907; transcription factor NRF
• UniProt Protein Name: NF-kappa-B-repressing factor
• Protein Family: NF-kappa-B-repressing factor
• UniProt Gene Name: NKRF
• UniProt Synonym Gene Names: ITBA4; NRF
• UniProt Entry Name: NKRF_HUMAN

NCBI Description

This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

NKRF: Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Protein type: RNA-binding; Nucleolus; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: endoplasmic reticulum; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent