Mouse anti-Human Trefoil Factor 3 Monoclonal Antibody | anti-TFF3 antibody

Trefoil Factor 3, NT (TFF3)

Cat. Num. AAA603663

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by subsequent thiophilic adsorption and size exclusion chromatography.
• Synonyms: Trefoil Factor 3; Monoclonal Antibody; NT (TFF3); Anti -Trefoil Factor 3; anti-TFF3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D811
• Specificity: Recognizes the N-terminus of human TFF3. Detects a band of ~9kD by Western Blot.
• Purity/Purification: Affinity Purified; Purified by subsequent thiophilic adsorption and size exclusion chromatography.
• Form/Format: Supplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide.

• Applicable Applications for anti-TFF3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation,, Immunocytochemistry and Immunohistochemistry.; Dilution: Western Blot: 0.5ug/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; Immunoprecipitation: 1ug/10e6 SW480 cells; Immunocytochemistry: 1-10ug/ml (2-5ug/ml for DAB staining); Immunohistochemistry: Paraffin sections; Optimal dilution determined by the researcher.
• Immunogen: Synthetic peptide corresponding to aa1-12 from the N-terminal of human Trefoil Factor 3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TFF3 antibody

Trefoil factors are secreted polypeptides charakterized by a conserved trefoil domain structure defined by 3 specific disulfide bonds. TFFs were originally identified in the gastrointestinal tract where they were reported to have protective and reparative functions. The reparative function is

Product Categories/Family for anti-TFF3 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 281485608
• NCBI GeneID: 7033
• NCBI Accession #: NP_003217.3
• NCBI GenBank Nucleotide #: NM_003226.3
• UniProt Accession #: Q07654; Q96NX0; Q9UDA5
• Molecular Weight: 8,641 Da
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3 (intestinal)
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3; polypeptide P1.B; OTTHUMP00000109348; OTTHUMP00000109349
• UniProt Protein Name: Trefoil factor 3
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI
• UniProt Entry Name: TFF3_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq]

Uniprot Description

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response