Rat anti-Mouse Entactin Monoclonal Antibody | anti-Nid1 antibody
Entactin (Nidogen)
Gene Names
Nid1; Nid; entactin; nidogen-1; A630025O17; entactin-1; Nid1
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Purified
Synonyms
Entactin; Monoclonal Antibody; Entactin (Nidogen); Anti -Entactin (Nidogen); anti-Nid1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4A46
Specificity
Recognizes mouse Entactin.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide, 50% glycerol. No stabilizing proteins added.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Nid1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Immunohistochemistry (Frozen sections): 1:25-1:200 using avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Western Blot: 1:100-1:1000
Optimum dilutions to be determined by researcher.
Western Blot: 1:100-1:1000
Optimum dilutions to be determined by researcher.
Immunogen
Partially purified preparation of laminin from EHS mouse tumor.
Hybridoma
X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat.
Preparation and Storage
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Nid1 antibody
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal lamina contain type IV collagen, laminins, heparan sulfate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein of ~150kD.
Product Categories/Family for anti-Nid1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
entactin
NCBI Official Synonym Full Names
nidogen 1
NCBI Official Symbol
Nid1
NCBI Official Synonym Symbols
Nid; entactin; nidogen-1; A630025O17; entactin-1; Nid1
NCBI Protein Information
nidogen-1; NID-1; entactin 1; Nidogen precursor (Entactin)
UniProt Protein Name
Nidogen-1
Protein Family
UniProt Gene Name
Nid1
UniProt Synonym Gene Names
Ent
UniProt Entry Name
NID1_MOUSE
Uniprot Description
Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region; basement membrane; basal lamina
Molecular Function: laminin-1 binding; collagen binding; proteoglycan binding; protein binding; extracellular matrix binding; laminin binding; calcium ion binding
Biological Process: extracellular matrix organization and biogenesis; cell-matrix adhesion; glomerular basement membrane development; cell adhesion
Research Articles on Nid1
1. Results suggest a non-structural role for nidogen 1 in wound repair.
2. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411.
3. structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5)
4. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone
5. Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development.
6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2
7. Three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene.
8. Nid1 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue
9. nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud
10. Laminin gamma3 chain, located in murine basement membranes, binds to nidogen
2. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411.
3. structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5)
4. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone
5. Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development.
6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2
7. Three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene.
8. Nid1 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue
9. nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud
10. Laminin gamma3 chain, located in murine basement membranes, binds to nidogen
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Product Notes
The Nid1 nid1 (Catalog #AAA603630) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Entactin (Nidogen) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Entactin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Immunohistochemistry (Frozen sections): 1:25-1:200 using avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:100-1:1000 Optimum dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Nid1 nid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Entactin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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