Mouse Lck, p561ck Monoclonal Antibody | anti-LCK antibody

Lck, p561ck (Lymphocyte-specific Tyrosine Kinase)

Cat. Num. AAA603187

• Gene Names: LCK; LSK; YT16; p56lck; pp58lck
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified from ascites by Protein A affinity chromatography.
• Synonyms: Lck; p561ck; Monoclonal Antibody; p561ck (Lymphocyte-specific Tyrosine Kinase); Anti -Lck; anti-LCK antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2B2.51
• Specificity: Recognizes a protein with Mr56kD, identified as p56lck, a member of the Src family of non-receptor tyrosine protein kinases. This monoclonal antibody is specific for p56lck and does not crossreact with other members of the Src protein kinase family. Species Reactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified from ascites by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide

• Applicable Applications for anti-LCK antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blotting, Immunoprecipitation, Kinase Assays. Antibody preparations from which the BSA has been omitted have been used successfully as a coating antibody in ELISA.,; Dilution: ELISA: 1:100-1:1000; Western Blotting: 1.0ug/ml with an incubation of 2 hours at RT; Immunoprecipitation: 2.0 ug/mg of protein lysate
• Immunogen: Recombinant protein corresponding to amino acid residues 1-255 of murine p56lck protein.
• Hybridoma: Produced by fusion between BALB/c mouse splenocytes and mouse myeloma p3-NS1-Ag4-1 cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-LCK antibody

p56lck is myristoylated at its N-terminus, then translocated to the plasma membrane where it is palmitoylated and targeted to lipid rafts. p56lck associates with several proteins including c-Cbl through its SH3 domain and the cell surface glycoproteins CD4 and CD8 through its N-terminal domain. p56lck is expressed predominantly in T-cells and its function is critical both for T-cell development in the thymus and activation of mature T-cells in the periphery by antigen. The activity of p56lck is regulated by phosphorylation of two conserved tyrosine residues, tyrosine 505 (equivalent to tyrosine 529 of c-Src) and tyrosine 394 (equivalent to tyrosine 418 of c-Src). p56lck activation can increase responsiveness of some T cell hybridomas to antigen. The phosphorylation status and the activity of p56lck is regulated by the tyrosine protein phosphatase CD45, a protein which is also known as the leukocyte common antigen (LCA). The gene for p56lck is located in a chromosomal region which is susceptible to breakage and translocation. Abnormalities in this chromosomal region are often observed in lymphomas and neuroblastomas.

Product Categories/Family for anti-LCK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 349702
• NCBI GeneID: 3932
• UniProt Accession #: P06239; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8; Q573B4
• Molecular Weight: 58,001 Da
• NCBI Official Full Name: LCK
• NCBI Official Synonym Full Names: lymphocyte-specific protein tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000228508; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck
• UniProt Protein Name: Tyrosine-protein kinase Lck
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LCK
• UniProt Entry Name: LCK_HUMAN

NCBI Description

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]

Uniprot Description

Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Oncoprotein; Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: pericentriolar material; extrinsic to internal side of plasma membrane; endocytic vesicle; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; CD8 receptor binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine phosphatase activity; antigen binding; protein phosphatase binding; protein kinase binding; ATPase binding; protein binding; protein-tyrosine kinase activity; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; B cell receptor signaling pathway; positive regulation of T cell receptor signaling pathway; hemopoiesis; positive regulation of gamma-delta T cell differentiation; aging; T cell differentiation; epidermal growth factor receptor signaling pathway; response to drug; caspase activation; regulation of lymphocyte activation; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular zinc ion homeostasis; regulation of defense response to virus by virus; regulation of cell proliferation; response to hydrogen peroxide; positive regulation of tyrosine phosphorylation of Stat5 protein; dephosphorylation; response to zinc ion; response to mechanical stimulus; release of sequestered calcium ion into cytosol; T cell costimulation; innate immune response; positive regulation of T cell activation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 22