Western Blot (WB)
(Western Blot: HEK293 cell lysate was probed with MBS603155 (0.1ug/ml). Arrows indicates Ad2 and Ad5 E1A gene products.)
Mouse Adenovirus, Types 2,5, Early Region Monoclonal Antibody
Adenovirus, Types 2,5, Early Region (E1A)
Applications
ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Adenovirus; Types 2; 5; Early Region; Monoclonal Antibody; Early Region (E1A); Anti -Adenovirus; anti-Adenovirus; Early Region antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4i25
Specificity
Recognizes the three adenovirus (Ad) 2 and 5 E1A gene products, Mr 36-50kD.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in sodium phosphate, pH 7.4, 0.1% sodium azide, 0.2% gelatin.
Applicable Applications for anti-Adenovirus, Types 2,5, Early Region antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Immunoprecipitation, Immunocytochemistry and Western Blot.
Dilution: Western Blot: 0.05-0.1ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0879-05 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: An independent laboratory has reported A0879-05 immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins.
Immunocytochemistry: An independent laboratory has reported thatA0879-05 immunostains Ad2 and Ad5 E1A gene products.
Dilution: Western Blot: 0.05-0.1ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0879-05 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: An independent laboratory has reported A0879-05 immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins.
Immunocytochemistry: An independent laboratory has reported thatA0879-05 immunostains Ad2 and Ad5 E1A gene products.
Immunogen
TrpE-Ad2 E1A fusion protein isolated from E. coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot: HEK293 cell lysate was probed with MBS603155 (0.1ug/ml). Arrows indicates Ad2 and Ad5 E1A gene products.)
Western Blot (WB)
(Western Blot: HEK293 cell lysate was probed with MBS603155 (0.1ug/ml). Arrows indicates Ad2 and Ad5 E1A gene products.)
Related Product Information for anti-Adenovirus, Types 2,5, Early Region antibody
The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Product Categories/Family for anti-Adenovirus, Types 2,5, Early Region antibody
Similar Products
Product Notes
The Adenovirus, Types 2,5, Early Region (Catalog #AAA603155) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adenovirus, Types 2,5, Early Region can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Suitable for use in ELISA, Immunoprecipitation, Immunocytochemistry and Western Blot. Dilution: Western Blot: 0.05-0.1ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0879-05 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system. Immunoprecipitation: An independent laboratory has reported A0879-05 immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins. Immunocytochemistry: An independent laboratory has reported thatA0879-05 immunostains Ad2 and Ad5 E1A gene products. Researchers should empirically determine the suitability of the Adenovirus, Types 2,5, Early Region for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenovirus, Types 2,5, Early Region, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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