Mouse Benzo[a]pyrenediol Epoxide Monoclonal Antibody | anti-Ephx2 antibody

Benzo[a]pyrenediol Epoxide (BPDE)

Cat. Num. AAA601642

• Applications: ELISA
• Purity: Affinity Purified; Purified IgG
• Synonyms: Benzo[a]pyrenediol Epoxide; Monoclonal Antibody; Benzo[a]pyrenediol Epoxide (BPDE); Anti -Benzo[a]pyrenediol Epoxide (BPDE); anti-Ephx2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6A485
• Specificity: Recognizes free BPDE and DNA adducts.
• Purity/Purification: Affinity Purified; Purified IgG
• Form/Format: Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

• Applicable Applications for anti-Ephx2 antibody: ELISA (EL/EIA)
• Application Notes: Can be used for immunopurification and for ELISA.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Ephx2 antibody

A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representitive PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.

Product Categories/Family for anti-Ephx2 antibody

Antibodies; Abs to Biochemicals

NCBI and Uniprot Product Information

• NCBI GI #: 12621098
• NCBI GeneID: 65030
• NCBI Accession #: NP_075225.1
• NCBI GenBank Nucleotide #: NM_022936.1
• UniProt Accession #: P80299
• Molecular Weight: 62,340 Da
• NCBI Official Full Name: bifunctional epoxide hydrolase 2
• NCBI Official Synonym Full Names: epoxide hydrolase 2, cytoplasmic
• NCBI Official Symbol: Ephx2
• NCBI Protein Information: bifunctional epoxide hydrolase 2; CEH; SEH; epoxide hydratase; soluble epoxide hydrolase; cytosolic epoxide hydrolase
• UniProt Protein Name: Bifunctional epoxide hydrolase 2
• Protein Family: Bifunctional epoxide hydrolase
• UniProt Gene Name: Ephx2
• UniProt Synonym Gene Names: CEH; SEH
• UniProt Entry Name: HYES_RAT

NCBI Description

catalyzes the hydrolysis of an epoxide to a glycol; plays a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

EPHX2: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

Protein type: Phosphatase (non-protein); Hydrolase; Cell adhesion; Lipid Metabolism - arachidonic acid; EC 3.1.3.76; EC 3.3.2.10

Cellular Component: cytoplasm; peroxisome; cytosol

Molecular Function: toxin binding; protein homodimerization activity; lipid-phosphate phosphatase activity; epoxide hydrolase activity; phosphoric monoester hydrolase activity; magnesium ion binding; lipid phosphatase activity; receptor binding

Biological Process: prostaglandin production during acute inflammatory response; cholesterol homeostasis; dephosphorylation; response to toxin; linoleic acid metabolic process; stilbene catabolic process; sensory perception of pain; inflammatory response; aromatic compound catabolic process; positive regulation of blood pressure; phospholipid dephosphorylation