Mouse anti-Human Carboxypeptidase A2 Monoclonal Antibody | anti-CPA2 antibody

Carboxypeptidase A2

Cat. Num. AAA601437

• Gene Names: CPA2; DmelCG11291; Phos11291
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Carboxypeptidase A2; Monoclonal Antibody; Anti -Carboxypeptidase A2; anti-CPA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8B5
• Specificity: Recognizes human CPA2. Recognizes the pro form, (aa 17 - 417) but not the activated
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS with 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-CPA2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western blot, Immunoprecipitation, and Direct ELISA.; Dilution: Western blot: 1-2ug/ml Using a colorimetric detection system, the detection limit for rhCPA2 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.; Immunoprecipitation: 25ug/ml; Direct ELISA: 0.5-1.0ug/ml
• Immunogen: NS0-derived recombinant human rhCPA2.
• Endotoxin Level: < 0.1 EU per 1ug of the antibody as determined by the LAL method.
• Hybridoma: NSO myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CPA2 antibody

Carboxypeptidase A2 (CPA2) is a pancreatic peptidase that cleaves C-terminal residues with aromatic side chains including Trp, Tyr and Phe. The 411 aa human CPA2 consists of a pro region (aa 17 to 112) and a mature chain (aa 113 to 417).

Product Categories/Family for anti-CPA2 antibody

Antibodies; Abs to Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 217416390
• NCBI GeneID: 1358
• NCBI Accession #: NP_001860.2
• NCBI GenBank Nucleotide #: NM_001869.2
• UniProt Accession #: Q9W272; Q53XS1; Q96A12; Q96QN3; Q9UCF1; A4D1M4; C9JIK1; P48052
• Molecular Weight: 47,030 Da
• NCBI Official Full Name: carboxypeptidase A2
• NCBI Official Synonym Full Names: carboxypeptidase A2 (pancreatic)
• NCBI Official Symbol: CPA2
• NCBI Official Synonym Symbols: DmelCG11291; Phos11291
• NCBI Protein Information: carboxypeptidase A2; OTTHUMP00000211315
• UniProt Protein Name: Carboxypeptidase A2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPA2
• UniProt Entry Name: CBPA2_HUMAN

NCBI Description

Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq]

Uniprot Description

CPA2: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq, Dec 2008]

Protein type: EC 3.4.17.15; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular region

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: vacuolar protein catabolic process; proteolysis