Rabbit anti-Human NDUFS8 Polyclonal Antibody | anti-NDUFS8 antibody

NDUFS8, ID (NDUFS8, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, NADH-ubiquinone oxidoreductase 23kD subunit, TYKY subunit)

Cat. Num. AAA6013614

• Gene Names: NDUFS8; TYKY; CI-23k; CI23KD
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NDUFS8; Polyclonal Antibody; ID (NDUFS8; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial; Complex I-23kD; NADH-ubiquinone oxidoreductase 23kD subunit; TYKY subunit); Anti -NDUFS8; anti-NDUFS8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NDUFS8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: NDUFS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human NDUFS8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUFS8 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NDUFS8 antibody detected the NDUFS8 protein (arrow).)

Immunohistochemistry (IHC)

(NDUFS8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFS8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NDUFS8 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505371
• NCBI GeneID: 4728
• NCBI Accession #: NP_002487.1
• NCBI GenBank Nucleotide #: NM_002496.3
• UniProt Accession #: O00217; Q0VDA8; B2RB86
• Molecular Weight: 23,705 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS8
• NCBI Official Synonym Symbols: TYKY; CI-23k; CI23KD
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; complex I-23kD; complex I 23kDa subunit; NADH-ubiquinone oxidoreductase 23 kDa subunit
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
• UniProt Gene Name: NDUFS8
• UniProt Synonym Gene Names: CI-23kD
• UniProt Entry Name: NDUS8_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

TYKY: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone. Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Belongs to the complex I 23 kDa subunit family.

Protein type: EC 1.6.99.3; EC 1.6.5.3; Mitochondrial; Oxidoreductase; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity; 4 iron, 4 sulfur cluster binding; metal ion binding; NADH dehydrogenase activity

Biological Process: mitochondrial respiratory chain complex I assembly; cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; response to oxidative stress

Disease: Leigh Syndrome