Rabbit anti-Human Osteonectin, aa272-286 Polyclonal Antibody | anti-SPARC antibody

Osteonectin, aa272-286 (ON, Basement Membrane Protein 40, BM40, BM-40, Secreted Acidic Cysteine-rich Glycoprotein, Secreted Protein Acidic and Rich in Cysteine, Secreted Protein Acidic Cysteine-rich, SPARC, SPRC)

Cat. Num. AAA6013455

• Gene Names: SPARC; ON
• Reactivity: Human
• Applications: ELISA
• Purity: Serum; Serum
• Synonyms: Osteonectin; aa272-286; Polyclonal Antibody; aa272-286 (ON; Basement Membrane Protein 40; BM40; BM-40; Secreted Acidic Cysteine-rich Glycoprotein; Secreted Protein Acidic and Rich in Cysteine; Secreted Protein Acidic Cysteine-rich; SPARC; SPRC); Anti -Osteonectin; anti-SPARC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes synthetic human Osteonectin (aa272-286), and recombinant human Osteonectin. Does not crossreact with human Osteopontin or human Bone Sialoprotein.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophilized powder. Reconstitute with 20ul or 100ul sterile ddH2O.

• Applicable Applications for anti-SPARC antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:3000
• Immunogen: Synthetic peptide corresponding to aa272-286, CFGIKQKDIDKDLVI, from human Osteonectin (GeneID 6678, Swiss-Prot P09486).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SPARC antibody

SPARC, also known as Osteonectin and basement membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage and morphogenetic processes such as modeling of extracellular matrix.

Product Categories/Family for anti-SPARC antibody

Antibodies; Abs to Osteonectin

NCBI and Uniprot Product Information

• NCBI GI #: 338325
• NCBI GeneID: 6678
• UniProt Accession #: P09486; Q6IBK4; D3DQH9
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: osteonectin
• NCBI Official Synonym Full Names: secreted protein, acidic, cysteine-rich (osteonectin)
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON
• NCBI Protein Information: SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to peptide hormone stimulus; response to gravity; receptor-mediated endocytosis; platelet activation; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii