Mouse anti-Human Parvovirus B19 Monoclonal Antibody
Parvovirus B19, Human
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified by Protein A affinity chromatography from ascites.
Synonyms
Parvovirus B19; Monoclonal Antibody; Human; Anti -Parvovirus B19; anti-Parvovirus B19 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q2041
Specificity
Recognizes human Parvovirus B19. Recognizes an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. The epitope has been mapped to aa328-344 of VP2 capsid protein. Staining is localized predominantly in the nucleus though the cytoplasmic staining may be observed.
Purity/Purification
Purified by Protein A affinity chromatography from ascites.
Form/Format
Supplied as a liquid in 0.2M PBS, pH 7.6, 0.09% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Parvovirus B19 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Dilution: ELISA: 1:200
Immunofluorescence: 1:200-1:400
Immunohistochemistry (Paraffin): 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended.
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:200
Immunofluorescence: 1:200-1:400
Immunohistochemistry (Paraffin): 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended.
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Immunogen
Native parvovirus B19 purified from human plasma.
Grade
Affinity Purified
Positive Control
Parvovirus positive patient sample
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Parvovirus B19 antibody
Parvovirus B19 infection is common among children and adults and is often asymptomatic. Parvovirus B19 infection can result in serious and occasionally fatal hematologic diseases in susceptible patients, and is the cause of "fifth disease", a highly contagious infection of childhood. In sickle cell disease and other hereditary and acquired hemolytic anemias, acute B19 infection results in transient aplastic crisis, in which hemoglobin levels precipitously fall, anemia becomes life threatening, and congestive heart failure may ensue. Parvovirus B19 can also infect the fetus causing severe anemia, congestive heart failure, and heath in utero or at birth.
Product Categories/Family for anti-Parvovirus B19 antibody
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Product Notes
The Parvovirus B19 (Catalog #AAA601255) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Parvovirus B19, Human reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Parvovirus B19 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Dilution: ELISA: 1:200 Immunofluorescence: 1:200-1:400 Immunohistochemistry (Paraffin): 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Parvovirus B19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parvovirus B19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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