Goat Orexin Receptor Type 1, 2 Polyclonal Antibody | anti-HCRTR1 antibody

Orexin Receptor Type 1, 2 (Ox-1-R, Ox1R, Hypocretin Receptor Type 1, HCRTR1/ Ox-2-R, Ox2R, Hypocretin Receptor Type 2, HCRTR2)

Cat. Num. AAA6012357

• Gene Names: HCRTR1; OX1R
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Orexin Receptor Type 1; 2; Polyclonal Antibody; 2 (Ox-1-R; Ox1R; Hypocretin Receptor Type 1; HCRTR1/ Ox-2-R; Ox2R; Hypocretin Receptor Type 2; HCRTR2); Anti -Orexin Receptor Type 1; anti-HCRTR1 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human HCRTR1. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-HCRTR1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 0.3-1ug/ml, observed in human brain (frontal cortex), mouse and rat brain lysates on ~48kD bands
• Immunogen: Synthetic peptide corresponding to C-YNFLSGKFREQFK, from HCRTR1, at the internal region of the protein (NP_001516.2, NP_001517.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.5ug/ml) staining of Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-HCRTR1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 222080095
• NCBI GeneID: 3061
• NCBI Accession #: NP_001516.2
• NCBI GenBank Nucleotide #: NM_001525.2
• UniProt Accession #: O43613; Q9HBV6; A8K3A6
• Molecular Weight: 47,536 Da
• NCBI Official Full Name: orexin receptor type 1
• NCBI Official Synonym Full Names: hypocretin (orexin) receptor 1
• NCBI Official Symbol: HCRTR1
• NCBI Official Synonym Symbols: OX1R
• NCBI Protein Information: orexin receptor type 1; ox1-R; ox-1-R; orexin receptor 1; orexin receptor-1; hypocretin receptor 1; hypocretin receptor-1; hypocretin receptor type 1
• UniProt Protein Name: Orexin receptor type 1
• Protein Family: Orexin receptor
• UniProt Gene Name: HCRTR1
• UniProt Synonym Gene Names: Ox-1-R; Ox1-R; Ox1R
• UniProt Entry Name: OX1R_HUMAN

NCBI Description

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]

Uniprot Description

HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide hormone binding; peptide binding

Biological Process: synaptic transmission; neuropeptide signaling pathway; feeding behavior; cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep