Mouse anti-Human CENPJ Monoclonal Antibody | anti-CENPJ antibody

CENPJ (Centromere Protein J, CENP-J, BM032, Centrosomal P4.1-associated Protein, CPAP, LAG-3-associated Protein, LAP, LYST-interacting Protein 1, LIP1, MCPH6, MGC131581, MGC131582, MGC142222, MGC142224)

Cat. Num. AAA6012216

• Gene Names: CENPJ; LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CENPJ; Monoclonal Antibody; CENPJ (Centromere Protein J; CENP-J; BM032; Centrosomal P4.1-associated Protein; CPAP; LAG-3-associated Protein; LAP; LYST-interacting Protein 1; LIP1; MCPH6; MGC131581; MGC131582; MGC142222; MGC142224); Anti -CENPJ (Centromere Protein J; anti-CENPJ antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 5D5
• Specificity: Recognizes human CENPJ.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: KYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQ

• Applicable Applications for anti-CENPJ antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml
• Immunogen: Partial recombinant corresponding to aa979-1089 from human CENPJ (NP_060921) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.21kD).)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged CENPJ is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-CENPJ antibody

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Product Categories/Family for anti-CENPJ antibody

Antibodies; Abs to Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 130980075
• NCBI GeneID: 55835
• NCBI Accession #: NP_060921.3
• NCBI GenBank Nucleotide #: NM_018451.4
• UniProt Accession #: Q9HC77; Q2KHM6; Q5T6R5; Q96KS5; Q9C067
• Molecular Weight: 153,000 Da
• NCBI Official Full Name: centromere protein J
• NCBI Official Synonym Full Names: centromere protein J
• NCBI Official Symbol: CENPJ
• NCBI Official Synonym Symbols: LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J
• NCBI Protein Information: centromere protein J; LAG-3-associated protein; LYST-interacting protein 1; LYST-interacting protein LIP1; LYST-interacting protein LIP7; centrosomal P4.1-associated protein
• UniProt Protein Name: Centromere protein J
• Protein Family: Centromere protein
• UniProt Gene Name: CENPJ
• UniProt Synonym Gene Names: CPAP; LAP; LIP1; CENP-J
• UniProt Entry Name: CENPJ_HUMAN

NCBI Description

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CENPJ: Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Defects in CENPJ are the cause of microcephaly primary type 6 (MCPH6). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4). SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation. Belongs to the TCP10 family.

Protein type: Transcription, coactivator/corepressor; Cell cycle regulation; Microtubule-binding

Chromosomal Location of Human Ortholog: 13q12.12

Cellular Component: centriole; centrosome; microtubule; cytosol; gamma-tubulin small complex

Molecular Function: tubulin binding; protein domain specific binding; protein binding; protein kinase binding

Biological Process: regulation of centriole replication; cell division; organelle organization and biogenesis; centriole replication; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule nucleation; microtubule polymerization

Disease: Microcephaly 6, Primary, Autosomal Recessive; Seckel Syndrome 4