Rabbit anti-Human TSC2 Polyclonal Antibody | anti-TSC2 antibody

TSC2, CT (Tuberin, Tuberous Sclerosis 2 Protein, TSC4)

Cat. Num. AAA6011837

• Gene Names: TSC2; LAM; TSC4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TSC2; Polyclonal Antibody; CT (Tuberin; Tuberous Sclerosis 2 Protein; TSC4); Anti -TSC2; anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human TSC2. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TSC2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 15aa from human TSC2 at C-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with TSC2 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-TSC2 antibody

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Product Categories/Family for anti-TSC2 antibody

Antibodies; Abs to Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 116256352
• NCBI GeneID: 7249
• NCBI Accession #: NP_000539.2
• NCBI GenBank Nucleotide #: NM_000548.3
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIQ7; B4DRN2; C9J378
• Molecular Weight: 200,608 Da
• NCBI Official Full Name: tuberin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4
• NCBI Protein Information: tuberin; tuberous sclerosis 2 protein
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4
• UniProt Entry Name: TSC2_HUMAN

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; Cell cycle regulation; GAPs; Nuclear receptor co-regulator; GAPs, misc.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: TSC1-TSC2 complex; Golgi apparatus; growth cone; cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; dendrite; caveola; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding

Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; regulation of insulin receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of cell cycle; protein heterooligomerization; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; cell projection organization and biogenesis; positive chemotaxis; protein import into nucleus; regulation of endocytosis; acute-phase response; protein kinase B signaling cascade; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; response to hypoxia; insulin receptor signaling pathway; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade

Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2