Sheep anti-Human CHD1L Polyclonal Antibody | anti-CHD1L antibody

CHD1L (Chromodomain-helicase-DNA-binding Protein 1-like, Amplified in Liver Cancer Protein 1, ALC1)

Cat. Num. AAA6011662

• Gene Names: CHD1L; ALC1; CHDL
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CHD1L; Polyclonal Antibody; CHD1L (Chromodomain-helicase-DNA-binding Protein 1-like; Amplified in Liver Cancer Protein 1; ALC1); Anti -CHD1L (Chromodomain-helicase-DNA-binding Protein 1-like; anti-CHD1L antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHD1L. Species sequence homology: Recombinant human CHD1 and rhCHD5 (3%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-CHD1L antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa759-879 from human CHD1L expressed in CHO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human CHD1L by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Human CHD1L Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for CHD1L at approximately 115kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-CHD1L antibody

CHD-1L is a 98-118kD member of the SNF2/RAD54 helicase family of proteins. It is expressed in hepatocytes, possesses ATPase activity, and likely promotes chromatin remodeling at sites of DNA damage. It also is considered an oncogene. CHD-1L upregulates ARHGEF9 trabscription, an action that promotes Cdc42 activity with accompanying filopodia formation and EMT. It also binds apoptosis-mediating Nur77, blocking its migration from nucleus-to-mitochondria. Human CHD-1L is 897aa in length. It contains a helicase ATP-binding domain aa58-223, a C-terminal helicase domain aa351-513, a coiled-coil region aa38-675 and one Macro domain that binds poly-ADP-ribose and targets DNA damage sites aa704-897. There are at least two Ser/Thr phosphorylation sites. There are multiple potential splice variants. One isoform contains an alternative start site at Met114, a second isoform possesses a deletion of aa43-246, a third isoform shows a three aa substitution for aa363-897, and a fourth isoform combines a 16aa insertion after 386aa with a five aa substitution for aa425-897. Over aa759-879, human CHD-1L shares 94% aa identity with mouse CHD-1L.

Product Categories/Family for anti-CHD1L antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 373432623
• NCBI GeneID: 9557
• NCBI Accession #: NP_001243267.1
• NCBI GenBank Nucleotide #: NM_001256338.1
• UniProt Accession #: Q86WJ1; Q53EZ3; Q5VXX7; Q6DD94; Q6PK83; Q86XH3; Q96HF7; Q96SP3; Q9BVJ1; Q9NVV8; A5YM64
• Molecular Weight: 100,984 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 1-like isoform 5
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 1-like
• NCBI Official Symbol: CHD1L
• NCBI Official Synonym Symbols: ALC1; CHDL
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 1-like; amplified in liver cancer 1; amplified in liver cancer protein 1
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 1-like
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD1L
• UniProt Synonym Gene Names: ALC1
• UniProt Entry Name: CHD1L_HUMAN

NCBI Description

This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Several alternatively spliced transcripts variants have been described for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CHD1L: DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; DNA-binding

Chromosomal Location of Human Ortholog: 1q12

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; ATPase activity; nucleotide binding; ATP binding

Biological Process: chromatin remodeling; DNA repair; response to DNA damage stimulus; DNA duplex unwinding