Mouse anti-Human OATP2, 8 Monoclonal Antibody
OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
SupernatantHybridoma culture supernatant
Synonyms
OATP2; 8; Monoclonal Antibody; 8 (Organic Anion Transporting Polypeptide 2; 8); Anti -OATP2; anti-OATP2; 8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5F260
Specificity
Recognizes OATP2 and OATP8 human organic anion transporters (90kD, 120kD, respectively, after SDS-PAGE). Recognizes antigen in transfected HEK293 cells. Does not recognize antigen in HepG2 or PLC cell lines.
Purity/Purification
Supernatant
Hybridoma culture supernatant
Hybridoma culture supernatant
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Applicable Applications for anti-OATP2, 8 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation.
Dilution: Immunohistochemistry (Paraffin sections): Requires microwave pretreatment. Incubate overnight at 4 degree C.
Immunofluorescence: Stains the sinusoidal (basolateral) membranes of human liver and hepatocelular carcinoma. Incubate for 1 hour at RT.
Western Blot: For optimal detection of the glycosylated antigen, sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37 degree C for 30 minutes.
Dilution: Immunohistochemistry (Paraffin sections): Requires microwave pretreatment. Incubate overnight at 4 degree C.
Immunofluorescence: Stains the sinusoidal (basolateral) membranes of human liver and hepatocelular carcinoma. Incubate for 1 hour at RT.
Western Blot: For optimal detection of the glycosylated antigen, sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37 degree C for 30 minutes.
Immunogen
Synthetic peptide corresponding to 24aa from the N-terminus of human OATP2 conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-OATP2, 8 antibody
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. The OATP family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Oatp2 (also called OATP-B1; 661 aa; 12 TM), cloned from rat brain, shares significant homology with rat oatp1 and OAT-K1 (77%) and human OATP (73%). It is highly expressed in brain, liver, and kidney but not in heart, spleen, lung, muscle, and testes. OATP2 mediates uptake of taurocholate, estrogen conjugates, and cardiac glycosides ouabain. Unlike other OATPS, OATP2 also transport digioxin.
Product Categories/Family for anti-OATP2, 8 antibody
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Product Notes
The OATP2, 8 (Catalog #AAA601066) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OATP2, 8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Dilution: Immunohistochemistry (Paraffin sections): Requires microwave pretreatment. Incubate overnight at 4 degree C. Immunofluorescence: Stains the sinusoidal (basolateral) membranes of human liver and hepatocelular carcinoma. Incubate for 1 hour at RT. Western Blot: For optimal detection of the glycosylated antigen, sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37 degree C for 30 minutes. Researchers should empirically determine the suitability of the OATP2, 8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OATP2, 8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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