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Western Blot (WB) (MPPE1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPPE1 antibody detected the MPPE1 protein (arrow).)

Rabbit anti-Human MPPE1 Polyclonal Antibody | anti-MPPE1 antibody

MPPE1, CT (MPPE1, PGAP5, Metallophosphoesterase 1, Post-GPI attachment to proteins factor 5)

Gene Names
MPPE1; PGAP5
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
MPPE1; Polyclonal Antibody; CT (MPPE1; PGAP5; Metallophosphoesterase 1; Post-GPI attachment to proteins factor 5); Anti -MPPE1; anti-MPPE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-MPPE1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
MPPE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human MPPE1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MPPE1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPPE1 antibody detected the MPPE1 protein (arrow).)

Western Blot (WB) (MPPE1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPPE1 antibody detected the MPPE1 protein (arrow).)
Related Product Information for anti-MPPE1 antibody
Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins.
Product Categories/Family for anti-MPPE1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,141 Da
NCBI Official Full Name
metallophosphoesterase 1 isoform 2
NCBI Official Synonym Full Names
metallophosphoesterase 1
NCBI Official Symbol
MPPE1
NCBI Official Synonym Symbols
PGAP5
NCBI Protein Information
metallophosphoesterase 1; metallo phosphoesterase; post-GPI attachment to proteins factor 5
UniProt Protein Name
Metallophosphoesterase 1
Protein Family
UniProt Gene Name
MPPE1
UniProt Synonym Gene Names
PGAP5
UniProt Entry Name
MPPE1_HUMAN

Uniprot Description

MPPE1: Metallophosphoesterase required for transport of GPI- anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins. Belongs to the metallophosphoesterase superfamily. MPPE1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.1.-.-; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18p11.21

Cellular Component: nucleoplasm; Golgi apparatus; ER-Golgi intermediate compartment membrane; cis-Golgi network; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: phosphoric diester hydrolase activity; manganese ion binding

Biological Process: ER to Golgi vesicle-mediated transport; GPI anchor biosynthetic process

Research Articles on MPPE1

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Product Notes

The MPPE1 mppe1 (Catalog #AAA6010590) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MPPE1, CT (MPPE1, PGAP5, Metallophosphoesterase 1, Post-GPI attachment to proteins factor 5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MPPE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the MPPE1 mppe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPPE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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