Goat anti-Mouse, Rat CIRBP Polyclonal Antibody | anti-CIRBP antibody

CIRBP (Glycine-rich RNA binding protein, Cold-inducible RNA-binding protein, Cold inducible RNA-binding protein, Cold inducible RNA binding protein, CIRP)

Cat. Num. AAA6010329

• Gene Names: CIRBP; CIRP
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CIRBP; Polyclonal Antibody; CIRBP (Glycine-rich RNA binding protein; Cold-inducible RNA-binding protein; Cold inducible RNA-binding protein; Cold inducible RNA binding protein; CIRP); Anti -CIRBP (Glycine-rich RNA binding protein; anti-CIRBP antibody

• Host: Goat
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes mouse CIRBP. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-CIRBP antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 0.01-0.03ug/ml
• Immunogen: Synthetic peptide corresponding to C-RDSYDSYATHNE, aa161-172 of mouse CIRBP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS6010329(0.01ug/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-CIRBP antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4502847
• NCBI GeneID: 1153
• NCBI Accession #: NP_001271.1
• NCBI GenBank Nucleotide #: NM_001280.2
• UniProt Accession #: Q14011
• Molecular Weight: 18,648 Da
• NCBI Official Full Name: cold-inducible RNA-binding protein
• NCBI Official Synonym Full Names: cold inducible RNA binding protein
• NCBI Official Symbol: CIRBP
• NCBI Official Synonym Symbols: CIRP
• NCBI Protein Information: cold-inducible RNA-binding protein; A18 hnRNP; glycine-rich RNA binding protein; cold inducible RNA-binding protein; glycine-rich RNA-binding protein CIRP
• UniProt Protein Name: Cold-inducible RNA-binding protein
• Protein Family: Cold-inducible RNA-binding protein
• UniProt Gene Name: CIRBP
• UniProt Synonym Gene Names: A18HNRNP; CIRP
• UniProt Entry Name: CIRBP_HUMAN

Uniprot Description

Function: Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor

By similarity. Promotes assembly of stress granules (SGs), when overexpressed. Ref.5 Ref.7

Subunit structure: Interacts with EIF4G1. Associates with ribosomes. Ref.7

Subcellular location: Nucleus › nucleoplasm. Cytoplasm. Note: Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins

By similarity. Ref.5

Tissue specificity: Ubiquitous.

Induction: By cold stress in response to DNA damage induced by UV irradiation or UV mimetic agents. Up-regulated by hypoxia. Ref.6

Domain: Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression

By similarity.

Post-translational modification: Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs

By similarity.Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation. Ref.7

Sequence similarities: Contains 1 RRM (RNA recognition motif) domain.