Rabbit Insig1 Polyclonal Antibody | anti-INSIG1 antibody

Insig1 (Insulin-induced Gene 1 Protein, INSIG-1, Immediate-early Protein CL-6, Insulin-induced Growth Response Protein CL-6, Cl-6)

Cat. Num. AAA6007803

• Gene Names: INSIG1; CL6; CL-6
• Reactivity: Bovine, Hamster, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Insig1; Polyclonal Antibody; Insig1 (Insulin-induced Gene 1 Protein; INSIG-1; Immediate-early Protein CL-6; Insulin-induced Growth Response Protein CL-6; Cl-6); Anti -Insig1 (Insulin-induced Gene 1 Protein; anti-INSIG1 antibody

• Host: Rabbit
• Reactivity: Bovine, Hamster, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat InSig-1 at ~30kD and its precursor (~50kD). Species Crossreactivity: human, mouse, hamster and bovine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-INSIG1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-4ug/ml
• Immunogen: Synthetic peptide corresponding to aa248 from rat Insig1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Jurkat cell lysate using MBS6007803.)

Related Product Information for anti-INSIG1 antibody

Insulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.

Product Categories/Family for anti-INSIG1 antibody

Antibodies; Abs to Insulin

NCBI and Uniprot Product Information

• NCBI GI #: 241982794
• NCBI GeneID: 3638
• NCBI Accession #: NP_938150.2
• NCBI GenBank Nucleotide #: NM_198336.2
• UniProt Accession #: O15503; Q53XW8; Q9BUV5; A4D2N1; A8K6L0
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: insulin-induced gene 1 protein isoform 2
• NCBI Official Synonym Full Names: insulin induced gene 1
• NCBI Official Symbol: INSIG1
• NCBI Official Synonym Symbols: CL6; CL-6
• NCBI Protein Information: insulin-induced gene 1 protein; INSIG-1 membrane protein
• UniProt Protein Name: Insulin-induced gene 1 protein
• Protein Family: Insulin-induced gene 1 protein
• UniProt Gene Name: INSIG1
• UniProt Synonym Gene Names: INSIG-1
• UniProt Entry Name: INSI1_HUMAN

NCBI Description

Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2009]

Uniprot Description

INSIG1: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Belongs to the INSIG family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding

Biological Process: cell proliferation; inner ear morphogenesis; triacylglycerol metabolic process; metabolic process; palate development; middle ear morphogenesis; negative regulation of fat cell differentiation; SREBP-mediated signaling pathway; cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process