Rat anti-Human Natriuretic Peptide, C-Type Monoclonal Antibody
Natriuretic Peptide, C-Type (CNP)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by Protein G affinity chromatography.
Synonyms
Natriuretic Peptide; C-Type; Monoclonal Antibody; C-Type (CNP); Anti -Natriuretic Peptide; anti-Natriuretic Peptide; C-Type antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H204
Specificity
Recognizes human CNP (aa98-126) and human CNP (aa24-126). Does not crossreact with recombinant human ANP or BNP. Species sequence homology: mouse and rat
Purity/Purification
Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5 mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Natriuretic Peptide, C-Type antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~20ng/well.
Western Blot: 1-2ug/ml detects human CNP peptides containing aa98-126. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing and reducing conditions.
Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~20ng/well.
Western Blot: 1-2ug/ml detects human CNP peptides containing aa98-126. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing and reducing conditions.
Immunogen
E. coli-derived recombinant protein corresponding to aa24-126 from human CNP precursor (Accession #P23582). Species sequence homology: mouse and rat.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Natriuretic Peptide, C-Type antibody
C-Type Natriuretic Peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49aa propeptide and a 53aa mature peptide. Additional cleavage of the mature peptide generates 29aa and 22aa peptides which have comparable activity in some assays. Within the 53aa mature CNP, human shares 96% aa sequence identity with mouse and rat.
Product Categories/Family for anti-Natriuretic Peptide, C-Type antibody
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Product Notes
The Natriuretic Peptide, C-Type (Catalog #AAA600754) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Natriuretic Peptide, C-Type (CNP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Natriuretic Peptide, C-Type can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~20ng/well. Western Blot: 1-2ug/ml detects human CNP peptides containing aa98-126. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the Natriuretic Peptide, C-Type for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Natriuretic Peptide, C-Type, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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