Western Blot (WB)
(DPH1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the DPH1 antibody detected the DPH1 protein (arrow).)
Rabbit anti-Human DPH1 Polyclonal Antibody | anti-DPH1 antibody
DPH1, CT (DPH1, DPH2L, DPH2L1, OVCA1, Diphthamide biosynthesis protein 1, DPH1 homolog, Diphthamide biosynthesis protein 2 homolog-like 1, Ovarian cancer-associated gene 1 protein)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(DPH1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the DPH1 antibody detected the DPH1 protein (arrow).)
Western Blot (WB)
(DPH1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the DPH1 antibody detected the DPH1 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]
Uniprot Description
DPH1: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. Belongs to the DPH1/DPH2 family. DPH1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: cytosol; nucleus
Biological Process: cell proliferation; cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification
Research Articles on DPH1
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Product Notes
The DPH1 dph1 (Catalog #AAA6007338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DPH1, CT (DPH1, DPH2L, DPH2L1, OVCA1, Diphthamide biosynthesis protein 1, DPH1 homolog, Diphthamide biosynthesis protein 2 homolog-like 1, Ovarian cancer-associated gene 1 protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the DPH1 dph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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