Western Blot (WB)
(Western blot analysis of CLM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CLM1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CLM1 Polyclonal Antibody | anti-CD300LF antibody
CLM1, NT (CD300LF, CD300F, CLM1, IGSF13, IREM1, NKIR, CMRF35-like molecule 1, CD300 antigen-like family member F, Immune receptor expressed on myeloid cells 1, Immunoglobulin superfamily member 13, NK inhibitory receptor, CD300f)
Gene Names
CD300LF; CLM1; NKIR; CLM-1; IREM1; CD300f; IREM-1; IgSF13
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CLM1; Polyclonal Antibody; NT (CD300LF; CD300F; IGSF13; IREM1; NKIR; CMRF35-like molecule 1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; Immunoglobulin superfamily member 13; NK inhibitory receptor; CD300f); Anti -CLM1; anti-CD300LF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-CD300LF antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
CLM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~92 amino acids from the N-terminal region of human CLM1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CLM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CLM1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CLM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CLM1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(CLM1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CLM1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CLM1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CLM1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CD300LF antibody
CLM1 is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP.
Product Categories/Family for anti-CD300LF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,335 Da
NCBI Official Full Name
CMRF35-like molecule 1
NCBI Official Synonym Full Names
CD300 molecule-like family member f
NCBI Official Symbol
CD300LF
NCBI Official Synonym Symbols
CLM1; NKIR; CLM-1; IREM1; CD300f; IREM-1; IgSF13
NCBI Protein Information
CMRF35-like molecule 1; NK inhibitory receptor; inhibitory receptor IREM1; CD300 antigen like family member F; CD300 antigen-like family member F; immunoglobin superfamily member 13; CD300 molecule like family member f; immunoglobulin superfamily member 13; immune receptor expressed on myeloid cells 1
UniProt Protein Name
CMRF35-like molecule 1
Protein Family
UniProt Gene Name
CD300LF
UniProt Synonym Gene Names
CD300F; CLM1; IGSF13; IREM1; NKIR; CLM-1; IREM-1; IgSF13
UniProt Entry Name
CLM1_HUMAN
NCBI Description
CD300LF is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP (INPP5D; MIM 601582) (Sui et al., 2004 [PubMed 15184070]; Alvarez-Errico et al., 2004 [PubMed 15549731]).[supplied by OMIM, Mar 2008]
Uniprot Description
IREM-1: Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Belongs to the CD300 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral; Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: plasma membrane; integral to membrane
Biological Process: immune system process
Research Articles on CD300LF
Similar Products
Product Notes
The CD300LF cd300lf (Catalog #AAA6006236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLM1, NT (CD300LF, CD300F, CLM1, IGSF13, IREM1, NKIR, CMRF35-like molecule 1, CD300 antigen-like family member F, Immune receptor expressed on myeloid cells 1, Immunoglobulin superfamily member 13, NK inhibitory receptor, CD300f) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CD300LF cd300lf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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