Rabbit NUP35 Polyclonal Antibody | anti-NUP35 antibody

NUP35 (Nucleoporin NUP53, 35kD Nucleoporin, Mitotic Phosphoprotein 44, MP-44, Nuclear Pore Complex Protein Nup53, Nucleoporin Nup35, MP44, NUP53)

Cat. Num. AAA6006012

• Gene Names: NUP35; MP44; NP44; NUP53
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NUP35; Polyclonal Antibody; NUP35 (Nucleoporin NUP53; 35kD Nucleoporin; Mitotic Phosphoprotein 44; MP-44; Nuclear Pore Complex Protein Nup53; Nucleoporin Nup35; MP44; NUP53); Anti -NUP35 (Nucleoporin NUP53; anti-NUP35 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NUP35. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-NUP35 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to aa50-100 of human NUP35.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using MBS6006012 at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NUP35 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and MBS6006012 (bottom left, right) at 5ug/ml.)

Related Product Information for anti-NUP35 antibody

Nucleoporin Nup35, or Mitotic phosphoprotein 44, is a nuclear protein known to be present on the nuclear pore complex and is tightly associated with the nuclear membrane and lamina. This RNA-associated protein belongs to the Nup53 family and has a MPPN (mitotic phosphoprotein N' end) domain. It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins. Though the exact function of NUP35 is yet to be revealed, it may function as a component of the nuclear pore complex (NPC) which are collectively referred to as nucleoporins (NUPs). It also may play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors, and may also play an important part in the association of MAD1, a transcriptional repressor with the NPC. NUP35 is widely expressed in most of the tissues.

Product Categories/Family for anti-NUP35 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 31982904
• NCBI GeneID: 129401
• NCBI Accession #: NP_612142.2
• NCBI GenBank Nucleotide #: NM_138285.3
• UniProt Accession #: Q8NFH5; Q4ZFZ9; Q53S95; Q8TDJ1
• Molecular Weight: 34,774 Da
• NCBI Official Full Name: nucleoporin NUP53
• NCBI Official Synonym Full Names: nucleoporin 35kDa
• NCBI Official Symbol: NUP35
• NCBI Official Synonym Symbols: MP44; NP44; NUP53
• NCBI Protein Information: nucleoporin NUP53; MP-44; nucleoporin Nup35; 35 kDa nucleoporin; mitotic phosphoprotein 44; nuclear pore complex protein Nup53
• UniProt Protein Name: Nucleoporin NUP53
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP35
• UniProt Synonym Gene Names: MP44; NUP53; MP-44
• UniProt Entry Name: NUP53_HUMAN

NCBI Description

This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. [provided by RefSeq, Jul 2008]

Uniprot Description

NUP35: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. Belongs to the Nup53 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; nuclear membrane; intracellular membrane-bound organelle; plasma membrane; nuclear envelope; nuclear pore

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport