Rabbit anti-Human VKORC1 Polyclonal Antibody | anti-VKORC1 antibody

VKORC1, NT (VKORC1, VKOR, Vitamin K epoxide reductase complex subunit 1, Vitamin K1 2,3-epoxide reductase subunit 1)

Cat. Num. AAA6005671

• Gene Names: VKORC1; VKOR; MST134; MST576; VKCFD2; EDTP308; IMAGE3455200
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: VKORC1; Polyclonal Antibody; NT (VKORC1; VKOR; Vitamin K epoxide reductase complex subunit 1; Vitamin K1 2; 3-epoxide reductase subunit 1); Anti -VKORC1; anti-VKORC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-VKORC1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: VKORC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of human VKORC1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VKORC1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the VKORC1 antibody detected the VKORC1 protein (arrow).)

Immunohistochemistry (IHC)

(VKORC1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VKORC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-VKORC1 antibody

Antibodies; Abs to Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 45827739
• NCBI GeneID: 79001
• NCBI Accession #: NP_996560.1
• NCBI GenBank Nucleotide #: NM_206824.1
• UniProt Accession #: Q9BQB6; Q6UX90; Q7Z2R4; A6NIQ6; B2R4Z6
• Molecular Weight: 18,235 Da
• NCBI Official Full Name: vitamin K epoxide reductase complex subunit 1 isoform 2
• NCBI Official Synonym Full Names: vitamin K epoxide reductase complex, subunit 1
• NCBI Official Symbol: VKORC1
• NCBI Official Synonym Symbols: VKOR; MST134; MST576; VKCFD2; EDTP308; IMAGE3455200
• NCBI Protein Information: vitamin K epoxide reductase complex subunit 1; phylloquinone epoxide reductase; vitamin K1 2,3-epoxide reductase subunit 1; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensitive)
• UniProt Protein Name: Vitamin K epoxide reductase complex subunit 1
• Protein Family: Vitamin K epoxide reductase complex
• UniProt Gene Name: VKORC1
• UniProt Synonym Gene Names: VKOR
• UniProt Entry Name: VKOR1_HUMAN

NCBI Description

Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

VKORC1: Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2). VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K. Defects in VKORC1 are a cause of coumarin resistance (CMRES). A condition characterized by partial or complete resistance to warfarin or other 4-hydroxycoumarin derivatives. These drugs are used as anti-coagulants for the prevention of thromboembolic diseases in subjects with deep vein thrombosis, atrial fibrillation, or mechanical heart valve replacement. Belongs to the VKOR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.1.4.1; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: vitamin-K-epoxide reductase (warfarin-sensitive) activity; quinone binding

Biological Process: cellular protein metabolic process; vitamin K metabolic process; drug metabolic process; blood coagulation; post-translational protein modification; peptidyl-glutamic acid carboxylation

Disease: Coumarin Resistance; Vitamin K-dependent Clotting Factors, Combined Deficiency Of, 2