Rabbit anti-Human, Mouse E6AP Polyclonal Antibody | anti-HUWE1 antibody

E6AP (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, Oncogenic Protein-associated Protein E6-AP, Human Papillomavirus E6-associated Protein, Renal Carcinoma Antigen NY-REN-54, UBE3A, EPVE6AP, HPVE6A)

Cat. Num. AAA6003293

• Gene Names: HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: E6AP; Polyclonal Antibody; E6AP (Ubiquitin-protein Ligase E3A; E6AP Ubiquitin-protein Ligase; Oncogenic Protein-associated Protein E6-AP; Human Papillomavirus E6-associated Protein; Renal Carcinoma Antigen NY-REN-54; UBE3A; EPVE6AP; HPVE6A); Anti -E6AP (Ubiquitin-protein Ligase E3A; anti-HUWE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human E6AP. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 30% glycerol.

• Applicable Applications for anti-HUWE1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:500-1:2500
• Immunogen: Synthetic peptide corresponding to human E6AP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HUWE1 antibody

Angelman Syndrome (AS) is a neurological disorder characterized by severe mental retardation, seizures and speech impairment, which results from the lack of a functional maternal copy of the E6-AP gene. E6-AP, or Angelman Syndrome-Associated protein, has been shown to interact with the progesterone receptor as a coactivator, enhancing the transcriptional activity of this steroid receptor. Interestingly, E6-AP (UBE3A) is also a member of the E3 ubiquitin-protein ligase family which plays a role in defining the substrate specificity of the ubiquitin-proteasome degradation system. For example, E6-AP can interact directly with the growth and tumor-suppressive protein, p53 or indirectly as part of a human papillomavirus type 16 or 18 E6 protein complex. This interaction promotes the degradation of p53 via the ubiquitin-proteosome pathway. These two functions of E6-AP, coactivation and degradation, are independent of one another. Furthermore, the coactivation function of E6-AP in AS patients remains intact and the ubiquitin-protease function of the protein was compromised. It is unclear, however, how this causes AS.

Product Categories/Family for anti-HUWE1 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 61676188
• NCBI GeneID: 10075
• NCBI Accession #: NP_113584.3
• NCBI GenBank Nucleotide #: NM_031407.6
• UniProt Accession #: Q7Z6Z7; O15029; Q4G2Z2; Q5H961; Q6P4D0; Q8NG67; Q9BUI0; Q9HCJ4; Q9NSL6; Q9P0A9
• Molecular Weight: 481,891 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase HUWE1
• NCBI Official Synonym Full Names: HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: HUWE1
• NCBI Official Synonym Symbols: MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
• NCBI Protein Information: E3 ubiquitin-protein ligase HUWE1; ARF-binding protein 1; URE-binding protein 1; BJ-HCC-24 tumor antigen; large structure of UREB1; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; upstream regulatory element-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9
• UniProt Protein Name: E3 ubiquitin-protein ligase HUWE1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: HUWE1
• UniProt Synonym Gene Names: KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1
• UniProt Entry Name: HUWE1_HUMAN

NCBI Description

This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic mental retardation. [provided by RefSeq, Aug 2013]

Uniprot Description

HUWE1: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6. Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. Belongs to the TOM1/PTR1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein monoubiquitination; protein polyubiquitination; base-excision repair; histone ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; cell differentiation

Disease: Mental Retardation, X-linked, Syndromic, Turner Type