Western Blot (WB)
(ZWILCH Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).)
Rabbit anti-Human, Mouse ZWILCH Polyclonal Antibody | anti-zwilch antibody
ZWILCH, CT (ZWILCH, Protein zwilch homolog)
Gene Names
zwilch; CG18639; CG18729; DmelCG18729; Zwilch
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ZWILCH; Polyclonal Antibody; CT (ZWILCH; Protein zwilch homolog); Anti -ZWILCH; anti-zwilch antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-zwilch antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
ZWILCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-508 amino acids from the C-terminal region of human ZWILCH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ZWILCH Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).)
Western Blot (WB)
(ZWILCH Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).)
Western Blot (WB)
(ZWILCH Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).)
Western Blot (WB)
(ZWILCH Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).)
Related Product Information for anti-zwilch antibody
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.
Product Categories/Family for anti-zwilch antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,442 Da
NCBI Official Full Name
zwilch
NCBI Official Symbol
zwilch
NCBI Official Synonym Symbols
CG18639; CG18729; DmelCG18729; Zwilch
NCBI Protein Information
CG18729 gene product from transcript CG18729-RA; CG18729-PA; zwilch; zwilch-PA
UniProt Protein Name
Protein zwilch
Protein Family
UniProt Gene Name
zwilch
UniProt Entry Name
ZWILC_DROME
Uniprot Description
Function: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin, Mad2 complexes and spindly/CG15415 onto kinetochores. Ref.4 Ref.5 Ref.6
Subunit structure: Component of the RZZ complex composed of rod, Zw10 and zwilch. Ref.4
Subcellular location: Chromosome › centromere › kinetochore. Note: Localizes to kinetochores and kinetochore microtubules. Ref.4
Disruption phenotype: Lagging chromosomes at anaphase and precocious sister chromatid separation upon activation of the spindle checkpoint. Ref.4
Sequence similarities: Belongs to the ZWILCH family.
Sequence caution: The sequence AAL13732.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on zwilch
1. Zwilch localizes to kinetochores and kinetochore microtubules in a manner identical to ZW10 and ROD.
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Product Notes
The zwilch zwilch (Catalog #AAA6001952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZWILCH, CT (ZWILCH, Protein zwilch homolog) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZWILCH can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the zwilch zwilch for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZWILCH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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