Rabbit anti-Human PATL1 Polyclonal Antibody | anti-PATL1 antibody

PATL1, CT (PATL1, Protein PAT1 homolog 1, PAT1-like protein 1, Protein PAT1 homolog b)

Cat. Num. AAA6001324

• Gene Names: PATL1; Pat1b; hPat1b
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PATL1; Polyclonal Antibody; CT (PATL1; Protein PAT1 homolog 1; PAT1-like protein 1; Protein PAT1 homolog b); Anti -PATL1; anti-PATL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PATL1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: PATL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496~526 amino acids from the C-terminal region of human PATL1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HL-60 cell line lysates (35ug/lane) using MBS6001324. PATL1 (arrow) was detected using MBS6001324.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded skeletal muscle using MBS6001324 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001324 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HL-60 cells using MBS6001324 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PATL1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 189217919
• NCBI GeneID: 219988
• NCBI Accession #: NP_689929.2
• NCBI GenBank Nucleotide #: NM_152716.2
• UniProt Accession #: Q86TB9; Q2TA86; Q6P166; Q8N9M6; Q8NI63; B3KXT9
• Molecular Weight: 86,850 Da
• NCBI Official Full Name: protein PAT1 homolog 1
• NCBI Official Synonym Full Names: protein associated with topoisomerase II homolog 1 (yeast)
• NCBI Official Symbol: PATL1
• NCBI Official Synonym Symbols: Pat1b; hPat1b
• NCBI Protein Information: protein PAT1 homolog 1; PAT1-like protein 1; protein PAT1 homolog b
• UniProt Protein Name: Protein PAT1 homolog 1
• Protein Family: Patellin
• UniProt Gene Name: PATL1
• UniProt Synonym Gene Names: Pat1b; hPat1b
• UniProt Entry Name: PATL1_HUMAN

Uniprot Description

PATL1: RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV). Belongs to the PAT1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: PML body; intracellular membrane-bound organelle; nuclear speck; cytosol; CCR4-NOT complex

Molecular Function: protein binding; RNA binding; poly(U) binding; poly(rG) binding

Biological Process: deadenylation-dependent decapping; gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay