Western Blot (WB)
(Western blot using anti-AGL (C-term) antibody (AP2402c) at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.)
Rabbit anti-Human AGL Polyclonal Antibody | anti-AOR_1_2146154 antibody
AGL, CT (AGL, GDE, Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase)
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
AGL; Polyclonal Antibody; CT (AGL; GDE; Glycogen debranching enzyme; Glycogen debrancher; 4-alpha-glucanotransferase; Oligo-1; 4-1; 4-glucantransferase; Amylo-alpha-1; 6-glucosidase; Dextrin 6-alpha-D-glucosidase); Anti -AGL; anti-AOR_1_2146154 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-AOR_1_2146154 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot1:100-500
Immunofluorescence: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot1:100-500
Immunofluorescence: 1:10-50
Immunogen
AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1487~1516 amino acids from the C-terminal region of human AGL.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot using anti-AGL (C-term) antibody (AP2402c) at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.)
Western Blot (WB)
(Western blot using anti-AGL (C-term) antibody (AP2402c) at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.)
Testing Data
(Expression of myc-GS causes wild type but not the ÄCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÄCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.)
Testing Data
(Expression of myc-GS causes wild type but not the ÄCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÄCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-AOR_1_2146154 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
108,704 Da
NCBI Official Full Name
Alpha-glucosidase
NCBI Official Symbol
AOR_1_2146154
NCBI Protein Information
alpha-glucosidase
UniProt Protein Name
Alpha-glucosidase
UniProt Gene Name
agdA
UniProt Synonym Gene Names
AGL
UniProt Entry Name
AGLU_ASPOR
Uniprot Description
Function: Hydrolyzes malto-oligosaccharides, but has a low activity toward soluble starch.
Catalytic activity: Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Induction: By maltose.
Sequence similarities: Belongs to the glycosyl hydrolase 31 family.
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Product Notes
The AOR_1_2146154 agda (Catalog #AAA6001080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGL, CT (AGL, GDE, Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Western Blot, Immunofluorescence, ELISA Dilution: ELISA: 1:1,000 Western Blot1:100-500 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the AOR_1_2146154 agda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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