Goat anti-Human CENTG3 Polyclonal Antibody | anti-AGAP3 antibody

CENTG3, CT (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 3, AGAP-3, CRAM-associated GTPase, CRAG, Centaurin-gamma-3, Cnt-g3, MR1-interacting Protein, MRIP-1, AGAP3)

Cat. Num. AAA6000738

• Gene Names: AGAP3; CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CENTG3; Polyclonal Antibody; CT (Arf-GAP with GTPase; ANK Repeat and PH Domain-containing Protein 3; AGAP-3; CRAM-associated GTPase; CRAG; Centaurin-gamma-3; Cnt-g3; MR1-interacting Protein; MRIP-1; AGAP3); Anti -CENTG3; anti-AGAP3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CENTG3. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-AGAP3 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-NPSAELHRSPSLL from human CENTG3 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with CENTG3 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-AGAP3 antibody

CENTG3, or M-RIP, is a 116kD protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Product Categories/Family for anti-AGAP3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119574414
• NCBI GeneID: 116988
• UniProt Accession #: Q96P47; Q59EN0; Q96RK3
• Molecular Weight: 95,044 Da
• NCBI Official Full Name: centaurin, gamma 3, isoform CRA_b
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
• NCBI Official Symbol: AGAP3
• NCBI Official Synonym Symbols: CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3; centaurin-gamma-3; centaurin, gamma 3; CRAM-associated GTPase; MR1-interacting protein; CRMP (collapsin response mediator protein) associated
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
• Protein Family: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
• UniProt Gene Name: AGAP3
• UniProt Synonym Gene Names: CENTG3; AGAP-3; CRAG; Cnt-g3; MRIP-1
• UniProt Entry Name: AGAP3_HUMAN

Uniprot Description

CENTG3: a member of the centaurin family of ADP-ribosylation factor directed (ARF) GTPase-activating proteins (GAPs). Protective against the pathogenesis of polyglutamine diseases. Mediates the nuclear translocation of the CENTG3-polyQ complexes and the formation of promyelocytic leukemia (PML) protein-associated nuclear bodies in reactive oxygen species (ROS) signaling. Promotes the degradation of polyQ proteins at PML nuclear bodies through the ubiquitin-proteasome pathway. Knockdown by small interfering RNA in neuronal cells consistently blocked the nuclear translocation of polyQ and enhanced polyQ-mediated cell death. Three alternatively-spliced isoforms have been described.

Protein type: GAPs, ARF; GAPs

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: GTPase activity; GTP binding; zinc ion binding; polyubiquitin binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein import into nucleus, translocation; small GTPase mediated signal transduction; positive regulation of GTPase activity