Mouse anti-Rat, Human Leu-Enkephalin Monoclonal Antibody
Leu-Enkephalin Mouse Monoclonal Antibody (NOC1)
Reactivity
Rat, Human
Applications
Immunohistochemistry, Immunocytochemistry, immunoassay, Radioimmunoassay
Synonyms
Leu-Enkephalin; Monoclonal Antibody; Leu-Enkephalin Mouse Monoclonal Antibody (NOC1); Enkephalin; endorphins; endorphin; Met5-enkephalin; Leu5-enkephalin; Met-enkephalin; Leu-enkephalin; anti-Enkephalin; anti-endorphins; anti-endorphin; anti-Met5-enkephalin; anti-Leu5-enkephalin; anti-Met-enkephalin; anti-Leu-enkephalin; anti-Leu-Enkephalin antibody
Host
Mouse
Reactivity
Rat, Human
Clonality
Monoclonal
Isotype
lgG1
Clone Number
NOC1
Specificity
A sensitive antibody against enkephalinergic fibrilar networks in the globus pallidus, N. accumbens, septum, hypothalamus, thalamus, amygdala, N. interstitialis of the stria terminalis and brain stem nuclei. This antibody does not distinguish between Met5-enkephalin and Leu5-enkephalin by radioimmunoassay or by immunocytochemistry. It is recommended for localizing enkephalinergic sites by immunohistochemistry. It does not bind to beta-endorphin or dynorphin-containing areas. It exhibits approximately. 40% cross reactivity with c-terminal extended Met-enkephalin hexapeptides and 7% cross reactivity with the extended heptapeptide (-Arg-Phe-OH), but does not recognize other endogenous peptides. All wellestablished enkephalin immunoreactive sites are recognized by this antibody in immunohistochemistry (IHC) and electron microscopy immunocytochemistry (ICC). Specific for: rat and human
Form/Format
Lyophilized tissue culture supernatant
Sequence Length
5
Applicable Applications for anti-Leu-Enkephalin antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Radioimmunoassay (RIA)
Application Notes
If reconstituted with deionized water in 200 µL: IHC / ICC 1:200 - 1:800. Optimal dilution has to be determined by the user.
Immunogen
Pentapeptide (Tyr-Gly-Gly-Phe-Leu)
Preservative
None
Target
Leu-enkephalin
Preparation and Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (please inquire). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period antibodies (please inquire) should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Related Product Information for anti-Leu-Enkephalin antibody
Enkephalins (penta-peptides) have been discovered as regulators of nerve impulses involving pain in the brain. It has been shown that these peptides act as natural analgesics (pain-killers) and their action mimics that of morphine and other opiates. At present, it is thought that the morphine-like effects are due to aromatic side chains on phenylanine and tyrosine which mimic a similar structure on morphine. Research suggests that it does not make much difference whether the enkephalin contains methionine or leucine at the acid end of the peptide for its action.
References
1. Bernacer J-Cholinergic interneurons are differentially distributed in the human striatum. 2. Andersson LI-Mimics of the binding sites of opioid receptors obtained by molecular imprinting of enkephalin and morphine. 3. Cuello AC-Characterization and immunocytochemical application of monoclonal antibodies against enkephalins. 4. Ma W-Substance P and enkephalin immunoreactivities in axonal boutons presynaptic to physiologically identified dorsal horn neurons. 5. Garzon M and Pickel VM-Ultrastructural localization of enkephalin and mu-opioid receptors in the rat ventral tegmental area.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Leu-enkephalin - blue mussel
Similar Products
Product Notes
The Leu-Enkephalin (Catalog #AAA588036) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Leu-Enkephalin Mouse Monoclonal Antibody (NOC1) reacts with Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Leu-Enkephalin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Radioimmunoassay (RIA). If reconstituted with deionized water in 200 µL: IHC / ICC 1:200 - 1:800. Optimal dilution has to be determined by the user. Researchers should empirically determine the suitability of the Leu-Enkephalin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leu-Enkephalin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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