Rat anti-Mouse CD200R3 Monoclonal Antibody | anti-CD200R3 antibody
CD200R3, Mouse, mAb Ba103, FITC
Gene Names
Cd200r3; AI505817; BB106877; mCD200RLb; 4733401I18Rik; 4833409J19Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD200R3; Monoclonal Antibody; Mouse; mAb Ba103; FITC; CD200 receptor-like 3; CD200RLb; cell surface glycoprotein OX-2 receptor 3; anti-CD200R3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
Ba103
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Sequence Length
205
Applicable Applications for anti-CD200R3 antibody
Flow Ctometry (FC/FACS), Western Blot (WB)
Application Notes
WB: reduced and non-reduced MC/9 lysate; band size 38kDa under reducing conditions and 74 kDa under nonreducing conditions
For flow cytometry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
For flow cytometry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Positive Control
MC/9 cells
Immunogen
Mouse primary basophils; cell-enriched bone marrow cells
Conjugate
FITC
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-CD200R3 antibody
The monoclonal antibody Ba103 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface, it is a single-pass type I membrane protein. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains, one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors function as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRg, or CD3z. CD200R3 is expressed in uterus and bone marrow-derived mast cells (at protein level). It is expressed in uterus, spleen, bone marrow-derived dendritic, basophil and mast cells. CD200R3 is also expressed in the lung of N. brasiliensis-infected mice. It is weakly expressed in brain, testis, lung and thymus. CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivo depletion of basophils by using monoclonal antibody Ba103 proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation. The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody Ba103 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba103 induces degranulation. Furthermore, monoclonal antibody Ba103 is useful for depletion of basophils.
References
1. Obata, K et al; Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood 2007, 110: 913 2. Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093
3. Khodoun, M et al; Peanuts can contribute to anaphylactic shock by activating complement. J Allergy Clin Immunol 2009, 123: 342
3. Khodoun, M et al; Peanuts can contribute to anaphylactic shock by activating complement. J Allergy Clin Immunol 2009, 123: 342
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,805 Da
NCBI Official Full Name
cell surface glycoprotein CD200 receptor 3 isoform 2
NCBI Official Synonym Full Names
CD200 receptor 3
NCBI Official Symbol
Cd200r3
NCBI Official Synonym Symbols
AI505817; BB106877; mCD200RLb; 4733401I18Rik; 4833409J19Rik
NCBI Protein Information
cell surface glycoprotein CD200 receptor 3; CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor-like 3; CD200RLb; cell surface glycoprotein OX2 receptor 3
UniProt Protein Name
Cell surface glycoprotein CD200 receptor 3
Protein Family
UniProt Gene Name
Cd200r3
UniProt Synonym Gene Names
Cd200rlb; CD200 receptor-like 3; CD200RLb
UniProt Entry Name
MO2R3_MOUSE
Uniprot Description
CD200R3: According to PubMed:15187158 isoform 4 is a receptor for the CD200 cell surface glycoprotein. According to PubMed:16081818 isoform 4 is not a receptor for the CD200/OX2 cell surface glycoprotein. Isoform 1, isoform 2 and isoform 3 are involved in the recruitment or surface expression of the TYROBP receptor. Isoform 6, isoform 7 and isoform 8 are not involved in the recruitment or surface expression of the TYROBP receptor. Belongs to the CD200R family. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity
Research Articles on CD200R3
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Product Notes
The CD200R3 cd200r3 (Catalog #AAA584681) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD200R3, Mouse, mAb Ba103, FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD200R3 can be used in a range of immunoassay formats including, but not limited to, Flow Ctometry (FC/FACS), Western Blot (WB). WB: reduced and non-reduced MC/9 lysate; band size 38kDa under reducing conditions and 74 kDa under nonreducing conditions For flow cytometry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the CD200R3 cd200r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD200R3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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