Human BPI ELISA Kit | BPI elisa kit

BPI, Human, ELISA Kit

Cat. Num. AAA584586

• Gene Names: BPI; rBPI; BPIFD1
• Reactivity: Human
• Synonyms: BPI; Human; ELISA Kit; BPI elisa kit

• Reactivity: Human
• Sequence Length: 487

• Application Notes: Application Notes: The human BPI ELISA Kit has been developed for the quantitative measurement of natural and recombinant human BPI in culture medium, plasma, wound fluid and broncheoalveolar lavage fluid. In plasma samples human BPI can be measured accurately if plasma samples are diluted at least 4 times.
• Detection Level: 250 pg/ml
• Standard Range: 100-25,000 pg/ml
• Working Volume: 100 ul/well
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for BPI elisa kit

Product Description: The antimicrobial protein BPI (Bactericidal Permeability Increasing protein) is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airway disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin. In plasma of healthy individuals BPI is present at levels of <0.5 ng/ml, which increases approximately 10-fold during acute phase responses.
Principle of the assay: The human BPI ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours.

References

1. Espinoza, J et al; Antimicrobial peptides in amniotic fluid: defensins, calprotectin and bacterial/permeability-increasing protein in patients with microbial invasion of the amniotic cavity, intra-amniotic inflammation, preterm labor and premature rupture of membranes. J Matern Fetal Neonatal Med 2003, 13: 2
2. Maris, N et al; Antiinflammatory effects of salmeterol after inhalation of lipopolysaccharide by healthy volunteers. Am J Respir Crit Care Med 2005, 172: 878
3. Gubern, C et al; Natural antibiotics and insulin sensitivity, the role of bactericidal/permeability-increasing protein. Diabetes 2006, 55: 216
4. Manco, M et al; Effect of massive weight loss on inflammatory adipocytokines and the innate immune system in morbidly obese women. J Clin Endocrinol Metab 2007, 92: 483
5. Strunk, T et al; Reduced levels of antimicrobial proteins and peptides in human cord blood plasma. Arch Dis Child Fetal Neonatal Ed 2009, 94: F230

NCBI and Uniprot Product Information

• NCBI GI #: 157276599
• NCBI GeneID: 671
• NCBI Accession #: NP_001716.2
• NCBI GenBank Nucleotide #: NM_001725.2
• Molecular Weight: 53,900 Da
• NCBI Official Full Name: bactericidal permeability-increasing protein
• NCBI Official Synonym Full Names: bactericidal/permeability-increasing protein
• NCBI Official Symbol: BPI
• NCBI Official Synonym Symbols: rBPI; BPIFD1
• NCBI Protein Information: bactericidal permeability-increasing protein; CAP 57; recombinant BPI holoprotein, rBPI; BPI fold containing family D, member 1
• UniProt Protein Name: Bactericidal permeability-increasing protein
• Protein Family: BPI fold-containing family
• UniProt Gene Name: BPI
• UniProt Synonym Gene Names: BPI
• UniProt Entry Name: BPI_HUMAN