Mouse anti-Human VCAM-1 Monoclonal Antibody | anti-VCAM-1 antibody

VCAM-1, Human, mAb 1G11B1

Cat. Num. AAA584431

• Gene Names: VCAM1; CD106; INCAM-100
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: VCAM-1; Monoclonal Antibody; Human; mAb 1G11B1; CD106; INCAM-100; L1CAM; MGC99561; DKFZp779G2333; anti-VCAM-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 739

• Applicable Applications for anti-VCAM-1 antibody: F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP)
• Application Notes: Application Notes: F: Cryostatic sections were fixed in acetone, and incubated with mAb at 10 ug/ml in PBS/0.1% tween20; Application Use: For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: Full length protein
• Positive Control: Activated endothelial cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-VCAM-1 antibody

Product Description: The monoclonal antibody 1G11B1 recognizes vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 is a member of the immunoglobulin superfamily of adhesion molecules, which includes ICAMs, PECAM-s and MADCAM, and is involved in leukocyte-endothelial cell interactions. The immunoglobulin superfamily is a type I transmembrane protein characterized by extracellular immunoglobin domains, a transmembrane region and a cytoplasmic tail. They are essential for the development of the embryo and for immune and inflammatory responses. These transmembrane glycoproteins mediate cell interaction with, and adhesion to, other cells and the extracellular matrix. VCAM-1 contains six immunoglobulin domains of the H-type and interacts with VLA-4 expressed on leukocytes. Multiple adhesion molecules play a role in leukocyte recruitment. The process of migration of a leukocyte through the vascular endothelium consists of the following steps: leukocyte-endothelium interaction (first tethering and rolling and than adhesion) and transendothelial migration. VCAM-1 is almost not expressed under physiological conditions. However, under appropriate pro-inflammatory conditions where the endothelium is exposed to inflammatory cytokines such as tumour necrosis factor-alpha or IL-1b and becomes activated, VCAM-1 gene expression is rapid elevated by the vascular endothelium. There is also a soluble form of VCAM-1 which is angiogenic and chemotactic for endothelial cells. sVCAM-1 is up-regulated in several disease states (eg, myocardial infarction, type 2 diabetes mellitus, primary antiphospholipid syndrome, and rheumatoid arthritis).

References

1. Thornhill, M et al; Tumor necrosis factor combines with IL-4 or IFN-gamma to selectively enhance endothelial cells adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms. J Immunology 1991, 146: 592 2. Van der Vieren, M. et al; The Leukocyte Integrin alpha4beta2- binds VCAM-1: Evidence for a binding interface between I domain and VCAM-1. J Immunol 1999, 163:1984
3. Dienst, A et al; Specific occlusion of murine and human tumor vasculature by VCAM-1-Targeted recombinant fusion protein. J Natl Cancer Inst 2005, 97 :733
4. Wijbrandt, C et al; The clinical response to infliximab in rheumatoid arthritis is in part dependent on pretreatment tumour necrosis factor ; expression in the synovium. Ann Rheum Dis 2008, 67:1139
5. Roussel, L et al; IL-17 Promotes p38 MAPK-dependent endothelial activation enhancing- neutrophil recruitment to sites of inflammation. J immunol. 2010, 184:4531

NCBI and Uniprot Product Information

• NCBI GI #: 137560
• NCBI GeneID: 7412
• Molecular Weight: 74,346 Da
• NCBI Official Full Name: Vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1; CD106 antigen
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_HUMAN

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; apical part of cell; early endosome; plasma membrane; integral to membrane; podosome; sarcolemma; external side of plasma membrane; filopodium

Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding

Biological Process: response to nicotine; regulation of immune response; chronic inflammatory response; extracellular matrix organization and biogenesis; viral reproduction; cytokine and chemokine mediated signaling pathway; heart development; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging