Mouse anti-Rat C6 Monoclonal Antibody | anti-C6 antibody
C6, Rat, mAb 3G11
Reactivity
Rat
Applications
Western Blot
Synonyms
C6; Monoclonal Antibody; Rat; mAb 3G11; anti-C6 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-C6 antibody
F, Immunoasssay (IA), Western Blot
Application Notes
Application Use: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-C6 antibody
Product Description: The monoclonal antibody 3G11 reacts specifically with rat C6. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 membrane attack complex. Complement component C6 is part of this C5b-9 complex. This complex is assembled from five precursor molecules in the serum. The final step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. The rat remnant kidney model had been widely used to identify mechanisms responsible for the progression of renal disease. Proteinuria is nephrotoxic and contributes to the progression of renal injury. Activation of complement in proteinuric urine results in tubular and interstitial damage. In C6-deficient rats, acute interstitial changes resolved despite ongoing proteinuria. This suggests that C6 mediates chronic progression of tubulointerstitial damage in rats with remnant kidneys. The monoclonal antibody 3G11 was raised by immunization of mice with C6 isolated from rat serum. Monoclonal antibody 3G11 can be used as detector in immuno assays detect C5b-9 in both serum and urine.
References
1. Schulze, M et al; Increased urinary excretion of C5b-9 distinguishes passive Heymann nephritis in the rat. Kidney Int 1989, 35: 60 2. Baker, P et al; Depletion of C6 prevents development of proteinuria in experimental membranous nephropathy in rats. Am J Pathol 1989, 135: 185
3. Sato, T et al; The terminal sequence of complement plays an essential role in antibody-mediated renal cell apoptosis. J Am Soc Nephrol 1999, 10: 1242
3. Sato, T et al; The terminal sequence of complement plays an essential role in antibody-mediated renal cell apoptosis. J Am Soc Nephrol 1999, 10: 1242
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Product Notes
The C6 (Catalog #AAA584423) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C6, Rat, mAb 3G11 reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C6 can be used in a range of immunoassay formats including, but not limited to, F, Immunoasssay (IA), Western Blot. Application Use: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the C6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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